Embryo isolation and staging were done as described previously4 (link). The immunofluorescence analysis and AP staining were performed essentially as described previously4 (link). The primary antibodies used were as follows: anti-AP2γ rabbit polyclonal, 1:500 (cat#sc-8977; Santa Cruz Biotechnology); anti-ETMT1/GLP mouse monoclonal, 5µg/mL (cat#PP-B0422-00; R&D Systems); anti-H3K9me2 mouse monoclonal, 1:500 (cat#ab1220; Abcam); anti-SOX2 goat polyclonal, 1:200 (cat#sc-17320, Santa Cruz Biotechnology). The following secondary antibodies from Molecular Probes (Eugene, OR) were used at a dilution of 1:500: Alexa Fluor 488 goat anti-rabbit IgG; Alexa Fluor 568 goat anti-mouse IgG. The stained embryos were mounted with VECTASHIELD Antifade Mounting Medium (cat#H-1000; VECTOR LABORATORIES). The immunofluorescence images and the AP staining images were taken by a confocal microscope (Zeiss LSM880) and a stereomicroscope (Leica M80), respectively. The image analyses were done by using ImageJ/Fiji software.
Anti h3k9me2 mouse monoclonal
Manufactured by Abcam
Anti-H3K9me2 mouse monoclonal is a laboratory reagent that can be used to detect the presence of the H3K9me2 histone modification. It is a mouse-derived monoclonal antibody specific for the histone H3 protein when dimethylated at lysine 9.
Automatically generated - may contain errors
Lab products found in correlation
Anti ap2γ rabbit polyclonal, by Santa Cruz Biotechnology (2 mentions)
Anti etmt1 glp mouse monoclonal, by R&D Systems (2 mentions)
Anti sox2 goat polyclonal, by Santa Cruz Biotechnology (2 mentions)
Alexa fluor 568 goat anti mouse igg, by Vector Laboratories (2 mentions)
Alexa fluor 488 goat anti rabbit igg, by Vector Laboratories (2 mentions)
Lsm 880, by Leica (2 mentions)
Vectashield antifade mounting medium, by Vector Laboratories (2 mentions)
2 protocols using anti h3k9me2 mouse monoclonal
1
Immunofluorescence Analysis of Embryo Markers
2
Immunofluorescence Analysis of Embryo Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Embryo isolation and staging were done as described previously4 (link). The immunofluorescence analysis and AP staining were performed essentially as described previously4 (link). The primary antibodies used were as follows: anti-AP2γ rabbit polyclonal, 1:500 (cat#sc-8977; Santa Cruz Biotechnology); anti-ETMT1/GLP mouse monoclonal, 5µg/mL (cat#PP-B0422-00; R&D Systems); anti-H3K9me2 mouse monoclonal, 1:500 (cat#ab1220; Abcam); anti-SOX2 goat polyclonal, 1:200 (cat#sc-17320, Santa Cruz Biotechnology). The following secondary antibodies from Molecular Probes (Eugene, OR) were used at a dilution of 1:500: Alexa Fluor 488 goat anti-rabbit IgG; Alexa Fluor 568 goat anti-mouse IgG. The stained embryos were mounted with VECTASHIELD Antifade Mounting Medium (cat#H-1000; VECTOR LABORATORIES). The immunofluorescence images and the AP staining images were taken by a confocal microscope (Zeiss LSM880) and a stereomicroscope (Leica M80), respectively. The image analyses were done by using ImageJ/Fiji software.
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