S monovette edta k2 tubes

Blood sampling for laboratory tests using S-Monovette-EDTA K2 tubes (Sarstedt AG & Co. KG, Nümbrecht, Germany) was limited to the timespan within 24 h after the onset of brain injury. Haematological parameters including total white cell count (WBC), red blood cell count (RBC), platelet count (PLT), differential white cell count (neutrophils, lymphocytes, monocytes, eosinophils, and basophils), and haemoglobin (HB) were determined by Sysmex XN-1000 (Europe Gmbh, Norderstedt, Germany). NLR, PLR, LMR, and SII were calculated and compared to references values according to Luo et al. [30 (link)]. The formulas are as follows: NLR = Neutrophil count (10³/µL)/Lymphocyte count (10³/µL); PLR = Platelet count (10³/µL)/Lymphocyte count (10³/µL); LMR = Lymphocyte count (10³/µL)/Monocyte count (10³/µL); SII = (Platelet count (10³/µL) × (Neutrophil count (10³/µL)/Lymphocyte count (10³/µL)).

Blood samples were taken from the median cubital vein using S-Monovette-EDTA K₂ tubes (Sarstedt, Austria) for flow cytometry analysis and morphology and S-Monovette - serum tubes were used for other biochemical markers. Serum samples were left to clot for 45 min before centrifugation and then centrifuged at 3000 g for 10 min. Aliquots of serum were stored at − 80 °C.

Blood samples were taken on the 1st day of the training camp (1 day before the first cryotherapy session), and then on the 9th day of the training camp (1 day after the last cryotherapy session) from the median cubital vein between 7.00 and 8.00 a.m. using S-Monovette-EDTA K₂ tubes (SARSTEDT AG & Co. KG, Nümbrecht, Germany) for hematological analysis and S-Monovette tubes for other biochemical markers. Within 20 min, the samples were centrifuged at 3000 g and +8 °C for 10 min. Aliquots of serum were stored at −80 °C. All samples were analyzed in duplicate or triplicate in a single assay to avoid interassay variability. The intraassay coefficients of variation (CV) for the used kits were <7%.