1100 liquid chromatography system

Manufactured by Agilent Technologies

Sourced in United States

The Agilent 1100 liquid chromatography system is a high-performance analytical instrument designed for the separation and detection of chemical compounds. It is capable of performing liquid chromatography, a widely used technique in analytical chemistry and biochemistry.

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Lab products found in correlation

Chemstation software, by Agilent Technologies (3 mentions) Zorbax sb c18 column, by Agilent Technologies (2 mentions) C18 column, by Waters Corporation (1 mentions) Uv 2401pc, by Shimadzu (1 mentions) Xevo tq s, by Waters Corporation (1 mentions) Autospec premier p776, by Waters Corporation (1 mentions) Silica gel, by Qingdao Haiyang Chemical (1 mentions) Agilent 6200 q tof ms system, by Agilent Technologies (1 mentions) Dad detector, by Agilent Technologies (1 mentions) Silica gel 60 f254, by Qingdao Marine Chemical (1 mentions) Av 500, by Bruker (1 mentions) Aviii 600, by Bruker (1 mentions)

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1100 system (48 citations) Agilent Liquid Chromatography System series 1100 (6 citations) Agilent 1100 high-performance liquid chromatography system (4 citations) 1100 high performance liquid chromatography system (4 citations) Liquid chromatography system (3 citations) Agilent 1100 series high-performance liquid chromatography system (3 citations) Agilent 1100 liquid chromatography system (3 citations) 1100 series capillary liquid chromatography system (2 citations)

5 protocols using 1100 liquid chromatography system

Docetaxel Quantification and Encapsulation

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Docetaxel concentrations were measured using a validated high pressure liquid chromatography-UV method43 (link) after liquid/liquid extraction and using a C18 column (25 cm×4.6 mm, 5 µm, Waters, Guyancourt, France). The mobile phase was composed of 53% of ammonium acetate buffer (35 nM, pH 5) and 47% of acetonitrile. Samples were eluted at a constant flow rate of 1.8 mL/min with UV detection (227 nm). Analysis was performed on an Agilent 1100 liquid chromatography system. Data were acquired and analyzed using Chemstation software (Agilent, Les Ulis, France). Docetaxel and paclitaxel typical retention times were 11 and 13.5 minutes, respectively. Encapsulation rate for docetaxel was calculated using the following formula:

Encapsulation rate = \frac{mg DOCE HPLC measured}{mg DOCE initially used} \times 100 .

Entrapment efficiency for docetaxel was calculated using the following formula:

Entrapment efficiency = \frac{mg DOCE HPLC measured}{mg DOCE present before centrifugation} \times 100 .

Rodallec A., Brunel J.M., Giacometti S., Maccario H., Correard F., Mas E., Orneto C., Savina A., Bouquet F., Lacarelle B., Ciccolini J, & Fanciullino R. (2018). Docetaxel–trastuzumab stealth immunoliposome: development and in vitro proof of concept studies in breast cancer. International Journal of Nanomedicine, 13, 3451-3465.

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Purification and Characterization of Cordycepin from CE-WIB801C

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The purification of cordycepin from CE-WIB801C was performed according to the Lee method (Lee et al., 2014c (link)). The methanol (50%) extract from CE-WIB801C was dissolved with 50% methanol and then purified by prep-HPLC. An Agilent 1100 liquid chromatography system (Palo Alto, CA, USA), equipped with vacuum degasser, quaternary gradient pump, autosampler and DAD, connected to an Agilent ChemStation software. A Jupiter C₁₈ column (250 mm×21.2 mm id, 5 μm) were used at a column temperature of 25°C. The mobile phase consisted of water (A) and methanol with 0.01M KH₂PO₄ (B) using the following program: 0-30 min, 15% B. The flow rate was at 25 mL/min and sample injection volume was 1.5 mL. The UV detection was operated at 254 nm. The purified cordycepin were freeze-dried using a freeze dryer (Clean-vac 24T, Biotron, Korea) to obtain powder, which were analyzed and calculated by analytic HPLC above condition. The cordycepin was dissolved in distilled water, and used to investigate the effects on platelet aggregation. In this study, the cordycepin from CE-WIB801C was called as W-cordycepin to differentiate from authentic cordycepin.

Lee D.H., Kim H.H., Lim D.H., Kim J.L, & Park H.J. (2015). Effect of Cordycepin-Enriched WIB801C from Cordyceps militaris Suppressing Fibrinogen Binding to Glycoprotein IIb/IIIa. Biomolecules & Therapeutics, 23(1), 60-70.

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Spectroscopic Characterization of Natural Products

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1D and 2D NMR spectra were obtained on a Bruker Avance III 600 MHz spectrometer (Bruker Biospin GmbH, Karlsruhe, Germany). HREIMS was measured on Waters Xevo TQ-S and Waters Autospec Premier P776 mass spectrometers (Waters, Milford, MA, USA). HRESIMS were recorded on an Agilent 6200 Q-TOF MS system (Agilent Technologies, Santa Clara, CA, USA). UV spectra were recorded on a Shimadzu UV-2401PC (Shimadzu, Kyoto, Japan). Optical rotations were obtained on a JASCO P-1020 digital polarimeter (Horiba, Kyoto, Japan). IR spectra were detected on Bruker Tensor 27 FTIR (KBr pellets) spectrometers. Sephadex LH-20 (Amersham Biosciences, Upssala, Sweden) and silica gel (Qingdao Haiyang Chemical Co., Ltd) were used for column chromatography (CC). Preparative high performance liquid chromatography (prep-HPLC) was performed on an Agilent 1100 liquid chromatography system equipped with Zorbax SB-C18 columns (9.4 mm × 250 mm) and a DAD detector (Agilent Technologies, Santa Clara, CA, USA). Thin-layer chromatography was performed on precoated TLC plates (200–250 μm thickness, silica gel 60 F254, Qingdao Marine Chemical, Inc.), and spots were visualized by heating after spraying.

Chu R., Wan L.S., Peng X.R., Yu M.Y., Zhang Z.R., Zhou L., Li Z.R, & Qiu M.H. (2016). Characterization of New Ent-kaurane Diterpenoids of Yunnan Arabica Coffee Beans. Natural Products and Bioprospecting, 6(4), 217-223.

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Comprehensive Analytical Workflow for Natural Products

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The NMR spectra were recorded on Bruker AV500 and AVIII600 instruments, with TMS used as an internal benchmark. Optical rotations were measured on an Autopol VI-91058 digital polarimeter. The UV spectra were detected by a UV-2700 spectrophotometer, while IR spectra were measured on a Nicolet iS10 spectrometer. HR-TOF ESI/MS was conducted on an Agilent 6,200 Q-TOF MS system spectrometer. Column chromatography was carried out with silica gel, reversed-phase C18 silica gel and Sephadex LH-20 as packing materials. Fractions were quantitated by thin-layer chromatography after spraying with sulphuric acid and heating. TLC was carried out on silica gel GF254. HPLC was performed on an Agilent 1,100 liquid chromatography system coupled with a diode-array detector and an Agilent Zorbax SB-C18 column (5 μm, 9.4 × 250 mm).

Mao F.F., Gao S.S., Huang Y.J., Zhou N., Feng J.K., Liu Z.H., Zhang Y.Q., Yuan L.Y., Wei G, & Cheng S.Q. (2023). Network pharmacology-based analysis of Resinacein S against non-alcoholic fatty liver disease by modulating lipid metabolism. Frontiers in Nutrition, 10, 1076569.

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HPLC Analysis of Nucleoside Analogues

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WIB801C was dissolved in 50% methanol, for the first time, and then it was analyzed by high performance liquid chromatography (HPLC). An Agilent 1100 liquid chromatography system (Palo Alto, CA., USA), equipped with vacuum degasser, quaternary gradient pump, autosampler and diode array detector, connected to an Agilent ChemStation software. A Zorbax octadecylsilane (ODS) C₁₈ column (250 mm×4.6 mm id, 5 μm) and a Zorbax ODS C₁₈ guard column (12.5 mm×4.6 mm id, 5 μm) were used at a column temperature of 25°C. The mobile phase consisted of water (A) and methanol with 0.01M KH₂PO₄ (B) using the following program: 0-30 min, 15% B. The flow rate was at 1.0 ml/min and sample injection volume was 10 μL. The UV detection was operated at 254 nm. To detect and analyze the nucleoside analogue, we used various concentrations (cordycepin: 50, 100, 200, and 400 μg/ml; adenosine: 4, 20, 100, and 200 μg/ml; adenine: 10, 20, 50, 100, 200 μg/ml) of each authentic compounds (cordycepin, adenosine, and adenine) in duplicate with HPLC, then the calibration curves were constructed by plotting the peak area against the concentration of each analyte with regression analysis, and we calculated linear equation from the calibration curve (Table 1).

Lee D.H., Kim H.H., Cho H.J., Yu Y.B., Kang H.C., Kim J.L., Lee J.J, & Park H.J. (2014). Cordycepin-Enriched WIB801C from Cordyceps militaris Inhibits Collagen-Induced [Ca2+]i Mobilization via cAMP-Dependent Phosphorylation of Inositol 1, 4, 5-Trisphosphate Receptor in Human Platelets. Biomolecules & Therapeutics, 22(3), 223-231.

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