U373mg

Human embryonic kidney cells (HEK293T) were obtained from Riken Cell Bank (Ibaraki, Japan). Human cancer cell lines U373MG (astrocytoma), H1299 (non‐small‐cell lung cancer), HCT116 (colorectal adenocarcinoma), and HBL100 (breast carcinoma) cells were purchased from ATCC (Rockville, MD, USA). Human mammary epithelial cells MCF10A p53^+/+ or MCF10A p53^−/− were purchased from Sigma Aldrich (St. Louis, MO, USA). HBC4 (breast carcinoma) cells were gifted from Dr. T. Yamori (Japanese Foundation for Cancer Research, Tokyo, Japan). HCT116 p53^+/+ and HCT116 p53^−/− cell lines were gifts from Dr. B. Vogelstein (Johns Hopkins University, Baltimore, MD, USA). HEK293T and U373MG cells were transfected with plasmids using Fugene6 (Promega, Madison, WI, USA), and Lipofectamin LTX (Invitrogen, Carlsbad, CA, USA), respectively. Small interfering RNA oligonucleotides, commercially synthesized by Sigma Genosys (Woollands, TX, USA), were transfected with Lipofectamine RNAiMAX reagent (Invitrogen). Sequences of siRNA oligonucleotides are shown in Table S1.

The human embryonic kidney cell line HEK293T was obtained from Riken Cell Bank. The human cancer cell lines U373 MG (astrocytoma), H1299 (non-small cell lung cancer), and HCT116 (colorectal adenocarcinoma) were purchased from American Type Culture Collection. Human mammary epithelial cells MCF10A p53^+/+ or MCF10A p53^−/− were purchased from Sigma Aldrich (St. Louis, MO, USA). HCT116 p53^+/+ and HCT116 p53^−/− cell lines were generously provided by B. Vogelstein (Johns Hopkins University, Baltimore, MD, USA). HEK293T and U373 MG cells were transfected with plasmids by using Fugene6 (Promega, Madison, WI, USA) and Lipofectamine LTX (Invitrogen, Carlsbad, CA, USA), respectively. Small interfering RNA (siRNA) oligonucleotides, which were commercially synthesized by Sigma Genosys, were transfected with Lipofectamine RNAiMAX reagent (Invitrogen). Sequences of the siRNA oligonucleotides used are shown in Supplementary Table 2.