Matrigel poor in growth factors

Manufactured by BD

Matrigel poor in growth factors is a cell culture matrix that provides a supportive and biologically relevant microenvironment for various cell types. It is a soluble basement membrane extract purified from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. This version of Matrigel is formulated with reduced levels of growth factors compared to the standard Matrigel product.

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Lab products found in correlation

Type 1 collagen, by BD (2 mentions) Hoechst, by Thermo Fisher Scientific (2 mentions) Cytoflex, by Beckman Coulter (2 mentions)

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Matrigel (17210 citations)

2 protocols using matrigel poor in growth factors

Evaluating MDSC Transwell Migration

Cited 1 time

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The upper wells of 8-µm-pore-size Boyden chambers were coated with MDSC stained with Hoechst (1:1,000 dilution, Thermo Fisher Scientific) at a density of 10³ cells/µL embedded in hydrogel composed by type 1 collagen (2 mg/mL, BD Bioscience) and Matrigel poor in growth factors (2 mg/mL, BD Bioscience) as previously described (58 (link)). HP was used at a concentration of 0.02 to 0.5 mg/mL based on the findings that murine serum levels are between 0.5 and 1.5 mg/mL in normal conditions. After 24 hours, cells were recovered from the lower chamber and were quantified by flow cytometry using a CytoFLEX (Beckman Coulter) and CyteExpert software. Experiments were repeated three times.

Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.

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Assessing MDSC Migration Using Boyden Chambers

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The upper wells of 8-μm pore size Boyden chambers were coated with MDSC stained with Hoechst (1:1000 dilution, ThermoFIsher Scientific) at a density of 10³ cells/μL embedded in hydrogel composed by Type 1 Collagen (2 mg/mL, BD Bioscience) and Matrigel poor in growth factors (2 mg/mL, BD Bioscience) as previously described (58 (link)). Haptoglobin was used at a concentration of 0.02-0.5 mg/ml based on the findings that murine serum levels are between 0.5-1.5 mg/ml in normal conditions. After 24 h, cells were recovered from the lower chamber and were quantified by flow cytometry using a CytoFLEX (Beckman Coulter) and CyteExpert software. Experiments were repeated 3 times.

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