Histone extracts from the hippocampus tissue (n = 3 per group) were prepared by using a total histone extraction kit (Epigentek) according to the manufacturer's protocol. Detection of global histone H3/H4 acetylation levels was measured using the EpiQuik™ global histone H3/H4 acetylation assay kit (Epigentek). Briefly, histone proteins (1–2 μg) were added to the strip wells. Acetylated histone H3/H4 was detected with a high‐affinity antibody, and the ratios and amounts of acetylated histone H3/H4 were displayed with a horseradish peroxidase‐conjugated secondary antibody color development system. The color was measured by reading absorbance at 450 nm using a Microplate Photometer.
Epiquik global histone h3 h4 acetylation assay kit
Manufactured by Epigentek
Sourced in United States
The EpiQuik™ global histone H3/H4 acetylation assay kit is a sensitive and reliable tool for quantifying the global levels of acetylated histone H3 and H4 proteins in various cell and tissue samples. The kit utilizes a unique and proprietary method to capture acetylated histones and detect them through a colorimetric reaction, providing a simple, fast, and high-throughput approach for histone acetylation analysis.
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Lab products found in correlation
2 protocols using epiquik global histone h3 h4 acetylation assay kit
1
Global Histone Acetylation Assay
2
Quantifying Histone Acetylation Levels
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Histone extracts from 12 samples (n = 4 per group) were prepared by using a total histone extraction kit (Epigentek) according to the manufacturer’s protocol. Detection of global histone H3/H4 acetylation status was performed using the EpiQuik™ global histone H3/H4 acetylation assay kit (Cat. P-4008-96/P-4009-96, Epigentek Group Inc., NY, USA), following the manufacturer’s recommendations. Briefly, histone proteins (1–2 μg) were added to the strip wells. Acetylated histone H3/H4 was detected with a high-affinity antibody, and the ratios and amounts of acetylated histone H3/H4 were displayed with a horseradish peroxidase-conjugated secondary anti-body color development system. The color was measured by reading absorbance at 450 nm using a Microplate Photometer.
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