Dulbecco s pbs 1

Manufactured by Merck Group

Dulbecco's PBS (1×) is a buffered saline solution commonly used in various laboratory applications. It serves as a base medium for cell culture, enzyme assays, and other biological procedures. The solution maintains a physiological pH and osmolarity, providing a suitable environment for a range of cellular and molecular activities.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Facscanto 2, by BD (1 mentions) Lsrfortessa, by BD (1 mentions)

Close spelling variants of this product

Dulbecco’s PBS Dulbecco PBS PBS 1×

2 protocols using dulbecco s pbs 1

Polymer Functionalization for Bioconjugation

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All
chemicals were used as received unless otherwise stated. Monomethoxy-poly(ethylene
glycol) (PEG) (1 kDa), monoamine-PEG (1 kDa), and monoazide-PEG (3
kDa) were purchased from JenKem Technology USA. Monomer trimethylene
carbonate was purchased from TCI Europe. BODIPY-FL carboxylic acid
was purchased from Lumiprobe. Immunoglobulin G, Human Fc Fragment
was purchased from Athenes Research Technology.
Dibenzocyclooctyne-amine-PEG₄-N-hydroxysuccinimide (DBCO-PEG₄-NHS) was purchased from Jena Bioscience. Amicon Ultra 0.5 mL 3 and
100 kDa spin filters were purchased from Sigma-Aldrich. CpG ODN 2006
and CpG ODN 2006-DBCO (5′ DBCO-PEG₄ modification)
were purchased from Biomers.net GmbH. All other chemicals and reagents,
including endotoxin-free Dulbecco’s PBS (1×) (w/o Ca⁺⁺ and Mg⁺⁺) were supplied by Sigma-Aldrich.

van Beek L.F., Welzen P.L., Teufel L.U., Joosten I., Diavatopoulos D.A., van Hest J, & de Jonge M.I. (2021). Bimodal Targeting of Human Leukocytes by Fc- and CpG-Decorated Polymersomes to Tune Immune Induction. Biomacromolecules, 22(10), 4422-4433.

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Multiparametric flow cytometry analysis

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Single cell suspensions of spleen, bone marrow (1 hind leg), peripheral lymph nodes as well as peritoneal cavity were prepared in Dulbecco’s PBS 1x (Sigma-Aldrich) with 2% fetal bovine serum (ThermoFisher). In addition, spleen and bone marrow preparations were treated with Gey’s solution to remove red blood cells. Cells were stained with the subsequent antibodies conjugated to APC, biotin, Bv421, Bv510, Bv605, Bv785, FITC, PE, PE-Cy7 or PerCP-Cy5.5: B220 (RA3-6B2), CD1d (1B1), CD11b (M1/70), CD19 (6D5), CD21 (7E9), CD23 (B3B4), CD25 (PC61), CD3ε (145-2C11), CD5 (53-7.3), CD62L (REA828), CD93 (AA4.1), c-Kit (2B8), hCD2 (RPA-2.10, TS1/8), IgD (11-26c.2a), IgM (Fab fragment and RMM-1), Itgα4 (CD49d; R1-2), Itgβ1 (CD29; HMβ1-1), LFA-1 (CD11a/CD18; H155-78), Ly6G (1A8), S1PR1 (713412) and TCRβ (H57-597) obtained from Biolegend, Jackson ImmunoResearch, Miltenyi, R&D systems and ThermoFisher. When required, samples were first stained with biotinylated antibodies followed by a second step with mixes including streptavidin coupled to an aforementioned fluorochrome and other labelled antibodies. Data were acquired on FACSCanto II and LSRFortessa flow cytometers (BD Biosciences) and analyzed using the FlowJo software (BD Biosciences).

Hagen M., Chakraborty T., Olson W.J., Heitz M., Hermann-Kleiter N., Kimpel J., Jenewein B., Pertoll J., Labi V., Rajewsky K, & Derudder E. (2022). miR-142 favors naïve B cell residence in peripheral lymph nodes. Frontiers in Immunology, 13, 847415.

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