Xba 1

Regarding the primary antibodies used in the study, anti-GAPDH (60004), anti-MYC(10828-1-AP), anti-LPCAT1 (66044, Mouse Monoclonal), and anti-SREBP1 (14088) were purchased from Proteintech (IL, USA); anti-MSMO1 (Thermo Fisher, USA), anti-SQLE, anti-LPCAT1 (ab214034, Rabbit Monoclonal), and anti-INSIG1 (ab70784, Rabbit polyclonal) were purchased from Abcam (Cambridge, UK); anti-phospho-SREBP1 (AF3283) was purchased from Affinity (OH, USA); anti-EGFR (#4267S), anti-phosphor-EGFR (#3777S), anti-PI3K (#4228), and anti-phosphor-PI3K (#4292) were purchased from Cell Signaling Technology (Danvers, PA USA). The restriction endonucleases (KpnI, Hind III, Nhe I, Xba I), KOD FX Neo were purchased from TOYOBO (SHANGHAI) BIOTECH CO., LTD. The Seamless Assembly Cloning Kit (CloneSmarter, USA) was purchased from Taihe Biotechnology Co., LTD. The activators 740 Y-P and NSC 228155 were purchased from MedChemExpress (NJ, USA).

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) was purchased from Cambridge Isotope Laboratory (Andover, MA, USA). Wizard Genomic DNA Purification kit and pGEM-T Easy Vector used in this study were from Promega (Madison, WI, USA). Oligonucleotides purified by HPLC were all purchased from Hokkaido System Science Co., Ltd. (Sapporo, Hokkaido, Japan). Restriction enzymes Nco I and Xba I were from Toyobo (Kita-ku, Osaka, Japan). Restriction enzyme HinP1 I was from New England BioLabs Inc. (Ipswich, MA, USA). Ligation Convenience kit was from Nippon Gene (Chiyoda-ku, Tokyo, Japan). PCR Purification kit and QIAquick Gel Extraction kit were from QIAGEN (Hilden, Germany). TaKaRa Ex Taq polymerase, RNA Transcript SureLABEL™ Core Kit, Solution I, and TaKaRa Hubble Slide Glass were from TaKaRa Bio (Kusatsu, Shiga, Japan). Big Dye Terminator v3.1 Cycle Sequencing kit was from ThermoFisher Scientific Inc. (San Diego, CA, USA). Cy3-UTP or Cy5-UTP were purchased from GE Healthcare UK Ltd. (Buckinghamshire, UK). OpHyb Hybridization buffer kit was from Operon Technologies (Alameda, CA, USA). LightCycler 480 SYBR Green I Master was from Roche Diagnostics Japan (Minato-ku, Tokyo, Japan).