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Amicron ultra

Manufactured by Merck Group
Sourced in United States

The Amicron Ultra is a versatile laboratory equipment designed for precise scientific measurements. It features advanced technology for high-accuracy performance. The core function of the Amicron Ultra is to provide reliable and consistent data collection for research and analytical applications.

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2 protocols using amicron ultra

1

Monoclonal Anti-ZP2 (114-129) Antibody Production

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A monoclonal murine ZP2 (aa 114-129) specific antibody (IE-3, rat IgG) was produced using the hybridoma cell line CRL-2463 (ATCC®, Manassas, Virginia, USA). Specific antibody purification was performed by affinity chromatography with HiTrap Protein G HP antibody purification columns (GE Healthcare, Little Chalfont, UK) according to the manufacturer’s instructions. Afterwards, the antibody solution was dialyzed with a pore size of 15.9 mm (Roth, Karlsruhe, Germany) overnight to increase purity and to neutralize the pH. After concentrating the antibody solution using 10 kDa Amicron Ultra centrifugal filters (Merck Millipore, Burlington, Massachusetts, USA) (2540 g, 20 min) and sterile filtrating (0.22 μM, Millex®-GV, Merck Millipore, Burlington, Massachusetts, USA), the optical density (OD) was determined. Specific binding of the antibody to N-terminal ZP2 (aa 114-129) was confirmed by ELISA and Western Blot analysis.
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2

Doxorubicin-Loaded Nano-Formulation for HER2+ Cancer

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Cancer cell lines BT-474, A549, Calu-3, and MCF-7 and media RPMI1640 were purchased from ATCC (Manassas, VA, USA). BT-474 is a HER2 positive breast cancer cell line; Calu-3 and A549 are HER2 positive lung cancer cell lines. MCF-7 is a HER2 negative breast cancer cell line. Non-cancerous human lung fibroblast cells (HLFs) and fibroblast basal medium were also purchased from ATCC (Manassas, VA, USA). Doxorubicin was purchased from Astatech. Lipid Dipalmitoylphosphatidylcholine (DPPC) (Avanti Polar Lipids Inc., Birmingham, AL, USA), Cholesterol (Millipore Sigma, Burlington, MA, USA), Poly(ethylene glycol) distearoylphosphatidylethanolamine (PEG-DSPE) (Lysan Bio. Inc., Arab, AL, USA) were purchased. Sephadex G-25 was from Millipore Sigma, CellTiter-Glo was from Promega, Amicron Ultra was from EMD Millipore, Spectrum™ Spectra/Por™ 1 RC dialysis membrane tubing (6000 to 8000 Dalton Molecular Weight cutoff (MWCO)) was from Fisher Scientific (Waltham, MA, USA). Athymic nude mice (Foxn1-nude, female, 6–7 weeks old) were purchased from ENVIGO Laboratories (Indianapolis, IN, USA). Luciferase transfected A549 cells (A549 Red-FLuc NSCLC cells) were from Perkin Elmer. Proximity ligation assay was performed using the Duolink® II assay kit (Sigma Aldrich, St. Louis, MO, USA). Primary antibodies for EGFR, HER2, and HER3 were from Enzo Life Sciences (Farmingdale, NY, USA).
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