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2 protocols using desipramine hcl

1

Dopaminergic Neurotransmission Assay

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All cell culture reagents except fetal bovine serum (FBS, Cambrex IEP GmbH (Wiesbaden, Germany)) were obtained from Gibco, Invitrogen (Paisley, UK). [3H]-dopamine (2220 GBq/mmol) was purchased from Perkin Elmer (Hopkinton, MA, USA), an E.Z.N.A.® Total RNA Kit I from Omega Bio-Tek (Norcross, GA, USA), and a High Capacity cDNA Reverse Transcription Kit, TaqMan Gene Expression Assays and TaqMan® Universal PCR Master Mix from Applied Biosystems (Carlsbad, CA, USA). Decynium-22 (D22), nortriptyline HCl, apomorphine HCl, haloperidol, L-DOPA, L-deprenyl HCl and tropolone were obtained from Sigma Aldrich (St. Louis, MO, USA), and desipramine HCl was obtained from Sandoz (Cham, Switzerland). GBR12909 was obtained from Tocris (Bristol, UK).
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2

Dopamine Transporter Regulation Assay

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All tissue culture reagents, except fetal bovine serum, which was from Cambrex IEP GmbH (Wiesbaden, Germany), were obtained from Gibco Invitrogen (Paisley, Scotland, UK). [3H]-DA (2220 GBq/mmol) was purchased from Perkin Elmer (Waltham, MA, USA). The E.Z.N.A.® HP Total RNA Kit was from Omega Bio-tek (Norcross, GA, USA), and the High Capacity cDNA Reverse Transcription Kit, TaqMan Gene Expression Assays, and TaqMan® Gene Expression Master Mix were from Applied Biosystems (Carlsbad, CA, USA). GBR12909 was from Tocris (Bristol, UK), and decynium 22 (D22), corticosterone, L-DOPA, haloperidol, and apomorphine HCl were obtained from Sigma Aldrich (St. Louis, MO, USA). Nortriptyline HCl, desipramine HCl, and amitriptyline HCl were purchased from Sandoz (Cham, Switzerland). The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) was obtained from Promega (Madison, WI, USA).
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