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Anti dpm1 antibody

Manufactured by Abcam

The Anti-Dpm1 antibody is a laboratory research tool that detects the Dpm1 protein. Dpm1 is a mannosyltransferase enzyme that plays a role in the synthesis of dolichol-phosphate-mannose, an essential component of protein glycosylation. This antibody can be used to study the expression and localization of Dpm1 in various cell and tissue samples.

Automatically generated - may contain errors

2 protocols using anti dpm1 antibody

1

Cell Surface Glycan Labeling Protocol

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Analytical-grade reagents were purchased from Sigma-Aldrich unless stated otherwise. Antibiotics were from Sigma-Aldrich, Invivogen or Invitrogen. Other reagents/materials were obtained as follows: D-2-[3H]-mannose (20–30 Ci/mmol) (American Radiolabeled Chemicals Inc.), Micro BCA protein assay kit (Thermo Fisher Scientific), Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare), anti-TAP antibody (Genescript), anti-Dpm1 antibody (Abcam), SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids). Yeast strains used in this study are listed in Table S5.
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2

Yeast Protein Purification Protocol

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Analytical-grade reagents were purchased from Sigma-Aldrich unless stated otherwise.
Antibiotics were from Sigma-Aldrich, Invivogen or Invitrogen. Other reagents/materials were obtained as follows: D-2-[ 3 H]-mannose (20-30 Ci/mmol) (American Radiolabeled Chemicals Inc.), Micro BCA protein assay kit (Thermo Fisher Scientific), Triton X-100 (Roche), IgG-Agarose resin (GE Healthcare), anti-TAP antibody (Genescript), anti-Dpm1 antibody (Abcam), SM-2 Biobeads and P6-Biogel resin (Biorad) and egg phosphatidylcholine (Avanti Polar Lipids). Yeast strains used in this study are listed in Table S5.
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