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Fcεriα af700

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FcεRIα-AF700 is a fluorescently labeled monoclonal antibody that binds to the alpha subunit of the high-affinity immunoglobulin E (IgE) receptor, FcεRI. This receptor is expressed on the surface of mast cells and basophils, and plays a crucial role in the initiation of the allergic response.

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Lab products found in correlation

Cd11b pe cy5, by BioLegend (2 mentions) Cd117 apc cy7, by BioLegend (2 mentions) Cd71 pe cy7, by BioLegend (2 mentions) Cd41 bv605, by BioLegend (2 mentions) Ter 119 bv421, by BioLegend (2 mentions) Ly 6g c fitc, by BD (2 mentions)

2 protocols using fcεriα af700

1

Erythroid Lineage Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) in the presence of 20% FCS supplemented with SCF (50 ng/mL), IL-3 (10 ng/mL), IL-6 (10 ng/mL), EPO (2 U/mL), TPO (50 ng/mL), IL-11 (50 ng/mL) and IL-5 (10 ng/mL) for 7 days. Cells were collected on days 2, 5 and 7, and labeled with the following cell surface markers for flow cytometric analysis:

TER119-BV421 (Clone TER-119, #116233 Biolegend)

CD71-PE Cy7 (Clone RI7217, #113812] Biolegend)

CD117-APC Cy7 (Clone 2B8, #105826 Biolegend)

FcεRIα-AF700 (Clone MAR-1,#134323 Biolegend)

CD41-BV605 (Clone MWReg30, #133921 Biolegend)

Cd11b-PE Cy5 (Clone M1/70, #101209 Biolegend)

Ly 6G/C-FITC (Clone RB6-8C5, #553126 BD Biosciences).

Tusi B.K., Wolock S.L., Weinreb C., Hwang Y., Hidalgo D., Zilionis R., Waisman A., Huh J.R., Klein A.M, & Socolovsky M. (2018). Population Snapshots Predict Early Hematopoietic and Erythroid Hierarchies. Nature, 555(7694), 54-60.
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2

Erythroid Lineage Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) in the presence of 20% FCS supplemented with SCF (50 ng/mL), IL-3 (10 ng/mL), IL-6 (10 ng/mL), EPO (2 U/mL), TPO (50 ng/mL), IL-11 (50 ng/mL) and IL-5 (10 ng/mL) for 7 days. Cells were collected on days 2, 5 and 7, and labeled with the following cell surface markers for flow cytometric analysis:

TER119-BV421 (Clone TER-119, #116233 Biolegend)

CD71-PE Cy7 (Clone RI7217, #113812] Biolegend)

CD117-APC Cy7 (Clone 2B8, #105826 Biolegend)

FcεRIα-AF700 (Clone MAR-1,#134323 Biolegend)

CD41-BV605 (Clone MWReg30, #133921 Biolegend)

Cd11b-PE Cy5 (Clone M1/70, #101209 Biolegend)

Ly 6G/C-FITC (Clone RB6-8C5, #553126 BD Biosciences).

Tusi B.K., Wolock S.L., Weinreb C., Hwang Y., Hidalgo D., Zilionis R., Waisman A., Huh J.R., Klein A.M, & Socolovsky M. (2018). Population Snapshots Predict Early Hematopoietic and Erythroid Hierarchies. Nature, 555(7694), 54-60.
+ Open protocol
+ Expand

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