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3h cholecystokinin 8 cck 8

Manufactured by PerkinElmer
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[3H]-cholecystokinin-8 (CCK-8) is a radiolabeled compound used in various research applications. It is a tritium-labeled version of the peptide hormone cholecystokinin-8, which plays a role in the regulation of digestive processes. The primary function of this product is to serve as a research tool for studying the biological activities and mechanisms associated with cholecystokinin-8.

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Lab products found in correlation

Bio safe 2 liquid scintillation mixture, by Research Products International (3 mentions) 3h estradiol 17 β d glucuronide e217βg, by PerkinElmer (2 mentions) E217βg, by Merck Group (2 mentions) Trypsin edta solution, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Triton x 100, by Merck Group (2 mentions) Antibiotic antimycotic solution, by Merck Group (2 mentions) Unlabeled cck 8, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Cytiva (2 mentions) Nicardipine, by Santa Cruz Biotechnology (1 mentions) Hank s balanced salt solution hbss, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Dulbecco s modified eagle medium, by Merck Group (1 mentions) Hepes, by BD (1 mentions) Geneticin, by BD (1 mentions) Vemurafenib, by LC Laboratories (1 mentions) 3h rosuvastatin, by American Radiolabeled Chemicals (1 mentions) 3h pitavastatin, by American Radiolabeled Chemicals (1 mentions) Dulbecco s modified eagle medium dmem, by Merck Group (1 mentions) Rifampicin, by Merck Group (1 mentions)

4 protocols using 3h cholecystokinin 8 cck 8

1

Receptor Binding Assay for E2 and CCK-8

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[3H]-estradiol 17 β-D-glucuronide (E217βG) (specific activity 37.2 Ci/mmol) and [3H]-cholecystokinin-8 (CCK-8) (specific activity 98.4 Ci/mmol) were purchased from Perkin Elmer Life Science (Waltham, MA, USA). Nicardipine was purchased from Santa Cruz Biotechnology (Dallas, TX, USA) Unlabeled CCK-8, E217βG, dimethyl sulfoxide (DMSO), Dulbecco’s Modified Eagle Medium (DMEM), Hank’s Balanced Salt Solution (HBSS), antibiotic antimycotic solution, trypsin–EDTA solution, Triton X-100, and fetal bovine serum (FBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Poly-L-lysine was purchased from Trevigen Inc. (Gaithersburg, MD, USA). Geneticin® and HEPES were purchased from BD Biosciences (Bedford, MA, USA). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL, USA).
Kayesh R., Tambe V., Xu C, & Yue W. (2023). Differential Preincubation Effects of Nicardipine on OATP1B1- and OATP1B3-Mediated Transport in the Presence and Absence of Protein: Implications in Assessing OATP1B1- and OATP1B3-Mediated Drug–Drug Interactions. Pharmaceutics, 15(3), 1020.
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2

Radioligand Binding Assay for CCK-8 and E2 17βG

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[3H]-Cholecystokinin 8 (CCK-8) (specific activity 88.0 Ci/mmol) and [3H]-estradiol 17 β-D-glucuronide (E217βG) (specific activity 41.4 Ci/mmol) were purchased from Perkin Elmer Life Science (Waltham, MA). Vemurafenib was purchased from LC laboratories (Woburn, MA, USA). Unlabeled CCK-8, E217βG, Triton X-100, dimethyl sulfoxide (DMSO), Hank’s Balanced Salt Solution (HBSS), Dulbecco’s Modified Eagle Medium (DMEM), trypsin-EDTA solution, and antibiotic antimycotic solution were purchased from Sigma-Aldrich (St. Louis, MO). Poly-L-lysine was purchased from Trevigen Inc. (Gaithersburg, MD). Geneticin® and HEPES was purchased from BD Biosciences (Bedford, MA). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, Utah). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL). Human plasma was purchased from BioIVT (Hicksville, NY, Lot#BRH895613).
Kayesh R., Farasyn T., Crowe A., Liu Q., Pahwa S., Alam K., Neuhoff S., Hatley O., Ding K, & Yue W. (2020). Assessing OATP1B1- and OATP1B3-mediated drug-drug interaction potential of vemurafenib using R-value and physiologically based pharmacokinetic models. Journal of pharmaceutical sciences, 110(1), 314-324.
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3

Characterization of Radiolabeled Compounds

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[ 3 H]cholecystokinin 8 (CCK-8) (specific activity 98.7.0 Ci/mmol) was purchased from Perkin Elmer Life Science (Waltham, MA). [ 3 H]Pitavastatin (specific activity 5 Ci/mmol), unlabeled pitavastatin and [ 3 H]rosuvastatin (specific activity 20 Ci/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO). Unlabeled rosuvastatin was purchased from Toronto Research Chemicals, Inc. (North York, ON, Canada). EVR, SIR, and dasatinib were purchased from LC laboratories (Woburn, MA). Unlabeled CCK-8, bromosulfophtalein (BSP), rifampicin, and Dulbecco's Modified Eagle Medium (DMEM) were purchased from Sigma-Aldrich (St. Louis, MO). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories (Logan, Utah). Bio-Safe II liquid scintillation mixture was purchased from Research Products International (Mt. Prospect, IL). All other materials were purchased from Thermo Fisher Scientific (Waltham, MA), Sigma-Aldrich (St. Louis, MO), or VWR International (Radnor, PA).
Farasyn T., Pahwa S., Xu C, & Yue W. (2021). Pre-incubation with OATP1B1 and OATP1B3 inhibitors potentiates inhibitory effects in physiologically relevant sandwich-cultured primary human hepatocytes. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 165.
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4

Pharmacokinetics of Labeled Compounds

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H]estradiol 17b-D-glucuronide, and [ 3 H]cholecystokinin-8 (CCK8) were obtained from PerkinElmer (Waltham, MA). [ 3 H]1-methyl-4phenylpyridinium and [ 14 C]metformin were obtained from American Radiolabeled Chemicals, Inc. Dabigatran and [ 14 C]Dabigatran were synthesized by Boehringer Ingelheim Pharma GmbH (Ingelheim, Germany). Apixaban, [ 13 C, 2 H 7 ]apixaban, and [ 13 C 6 ]Rivaroxaban were purchased from Alsachim (Illkirch-Graffenstaden, France). Rivaroxaban was purchased from Alsachim for human transporter studies and from ChemScene (Monmouth Junction, NJ) for rodent transporter studies. All other chemicals were of the highest reagent grade available from commercial sources.
Tsuruya Y., Nakanishi T., Komori H., Wang X., Ishiguro N., Kito T., Ikukawa K., Kishimoto W., Ito S., Schaefer O., Ebner T., Yamamura N., Kusuhara H, & Tamai I. (2017). Different Involvement of OAT in Renal Disposition of Oral Anticoagulants Rivaroxaban, Dabigatran, and Apixaban. Journal of pharmaceutical sciences, 106(9).
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