Recombinant murine il 11

Manufactured by Thermo Fisher Scientific

Recombinant murine IL-11 is a cytokine protein produced in E. coli cells. It is a member of the interleukin-6 family of cytokines and is involved in the regulation of immune and inflammatory responses.

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IL-11 (41 citations)

2 protocols using recombinant murine il 11

Hematopoietic Stem Cell Culture Conditions

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For growth assays, sorted CD34^-CD150⁺LSK HSCs were cultured in S-Clone SF-O3 (Sanko Junyaku) supplemented with 0.1% BSA (093001; STEMCELL Technologies), 50 μM 2-ME (Sigma-Aldrich) and 1% penicillin/streptomycin/glutamine (Gibco). 20 ng/mL of recombinant mouse SCF (579706; BioLegend) and recombinant human TPO (763706; BioLegend) for HSC culture conditions and 10 ng/mL of SCF, TPO, recombinant mouse IL-3 (575506; BioLegend), and recombinant murine GM-CSF (315-03; PeproTech) for myeloid culture condition-1 were added to cultures. In the case of myeloid culture condition-2, sorted CD150⁺CD48^-CD135^-CD34^-LSK HSCs, LSK cells, and GMPs were cultured in IMDM (Gibco) supplemented with 5% FBS, 50 μM 2-ME (Sigma-Aldrich), 1% penicillin/streptomycin/glutamine (Gibco), 1 mM sodium pyruvate (Gibco), and 0.1 mM MEM Non-Essential Amino Acids solution (Gibco). 25 ng/mL of SCF, TPO, recombinant human Flt3L (300-19; PeproTech) and recombinant murine IL-11 (220-11; PeproTech) and 10 ng/mL of IL-3 and GM-CSF were added to cultures.
For replating assays, LSK cells were plated in methylcellulose medium (Methocult M3234; STEMCELL Technologies) containing 20 ng/mL of SCF, TPO, IL-3, and GM-CSF.

Nakajima-Takagi Y., Oshima M., Takano J., Koide S., Itokawa N., Uemura S., Yamashita M., Andoh S., Aoyama K., Isshiki Y., Shinoda D., Saraya A., Arai F., Yamaguchi K., Furukawa Y., Koseki H., Ikawa T, & Iwama A. (2023). Polycomb repressive complex 1.1 coordinates homeostatic and emergency myelopoiesis. eLife, 12, e83004.

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Clonal analysis of mouse hematopoietic stem cells

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Freshly collected mouse BM was labeled according to panels exemplified in Supplementary Data. Single HSCs (LSK⁺CD34⁻Flt3⁻CD150⁺CD48⁻) were sorted into 96-well plates, using an Aria FACS Fusion II with a 100-μm nozzle. Cells were cultured for 10 days in IMDM-10% (see the “Cell culture” section), supplemented with SCF (50 ng/ml; recombinant murine SCF, PeproTech, #250-03), interleukin-3 (IL-3; 10 ng/ml; recombinant murine IL-3, PeproTech, #213-13), IL-6 (10 ng/ml; recombinant murine IL-6, PeproTech, #216-16), erythropoietin (EPO; 2 U/ml; BioLegend, #587602), IL-11 (50 ng/ml; recombinant murine IL-11, PeproTech, #220-11), IL-5 (10 ng/ml; recombinant murine IL-5, PeproTech, #215-15), thrombopoietin (TPO; 50 ng/ml; recombinant murine TPO, PeproTech, #315-14), IL-4 (10 ng/ml; recombinant murine IL-4, PeproTech, #214-14), granulocyte-macrophage colony-stimulating factor (GM-CSF; 15 ng/ml; recombinant murine GM-CSF, PeproTech, #315-03), and IL-7 (10 ng/ml; recombinant murine IL-7, PeproTech, #217-17). Growth factors/cytokines were refreshed at day 5. The clones/colonies in each well were labeled on day 10 with anti-CD117, anti-Ter119, anti-CD11b, and anti-IgD antibody. Clones were analyzed by flow cytometry using the BD Fortessa II (BD Biosciences) and processed with FlowJo 10.v.

Pessoa Rodrigues C., Herman J.S., Herquel B., Valsecchi C.I., Stehle T., Grün D, & Akhtar A. (2020). Temporal expression of MOF acetyltransferase primes transcription factor networks for erythroid fate. Science Advances, 6(21), eaaz4815.

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