Depigmentation was documented at 5 week age intervals using a flatbed scanner (Hewlett-Packard Company, Palo Alto, CA) and Adobe Software (Adobe Systems, Inc., San Jose, CA). Depigmentation was calculated as described (Denman et al., 2008 (link)). Briefly, anesthetized mice were placed on a flatbed scanner and resulting images were subjected to image analysis using Adobe Photoshop. Depigmentation was calculated from the largest evaluable area as the percentage of pixels among >150,000 evaluated with a luminosity above the cutoff level set to include 95% of pixels for untreated mice. Different animals were used to quantify depigmentation at each time point with the exception of Vitesse mice beyond 30 weeks of age. These later time points were included to evaluate when depigmentation tapers off in both groups. As relevant, mice were photographed using a DSC-S950 SteadyShot digital camera (Sony, Tokyo, Japan).
Flatbed scanner
Manufactured by Adobe
A flatbed scanner is a type of input device that captures images or text by placing the item to be scanned on a flat surface. It uses a light source and optical sensors to digitize the contents and convert them into a digital format, typically an image file.
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Lab products found in correlation
2 protocols using flatbed scanner
1
Quantifying Depigmentation in Mice
2
Quantitative 5-FOA Plasmid-Loss Assay
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The qualitative 5-FOA plasmid-loss assay was performed based on a previously described method and further adapted to obtain quantitative results (17 (link)). For the qualitative assay, yeast grown on SC-Ura, Leu solid medium were patched onto YPD solid medium and incubated for 2 to 3 days. The resultant patches were replica plated onto 5-FOA solid medium and incubated for 2 to 4 days. YPD and 5-FOA plates were imaged with a Canon flat-bed scanner (300 dpi), and images were equivalently processed using Adobe Photoshop for minor rotation, crop, and contrast adjustment. For the quantitative assay, the yeast described above were cultured in SC-Ura, Leu liquid medium for 2 days (A600 of ~2 to 3) and diluted with fresh medium, and a portion of the diluted culture estimated to have ~300 CFU was plated onto YPD solid medium and incubated for 2 to 3 days. Colonies on YPD solid medium were counted and then replica plated onto 5-FOA solid medium, and colonies on 5-FOA medium were counted after 2 to 4 days. For each condition, the ratio of colony numbers on 5-FOA medium to those on YPD was calculated and expressed as a percentage relative to the positive-control Ras2-CIIS (i.e., 100% reference condition).
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