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Pmir rb report luciferase reporter plasmid

Manufactured by RiboBio
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Sourced in China

The PmiR-RB-REPORT luciferase-reporter plasmid is a molecular biology tool designed for the analysis of microRNA (miRNA) target gene regulation. The plasmid contains a luciferase reporter gene that can be used to measure the activity of miRNA-target interactions.

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Lab products found in correlation

Dual luciferase reporter assay system, by Promega (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter system, by Promega (1 mentions)

Spelling variants of this product

PmiR-RB-REPORT (87 citations) PmiR-RB-REPORT™ plasmid (13 citations) PMIR-REPORT (10 citations) Luciferase reporter plasmid (6 citations)

2 protocols using pmir rb report luciferase reporter plasmid

1

Assessing miR-206 Binding to A3AR mRNA

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Wild-type (wt) and mutant (mut) putative miR-206-binding sites in the 3′-UTR of human A3AR mRNA, termed pmiR-A3AR-wt and pmiR-A3AR-mut, respectively, were generated through PCR-amplification and cloning into the pmiR-RB-REPORT luciferase-reporter plasmid (RiboBio). HT-29 cells were cotransfected with 500 ng of pmiR-A3AR-wt or pmiR-A3AR-mut plus 150 nM miR-206 mimic/inhibitor or their NCs by using Lipofectamine 2000. After 48-h incubation, luciferase activity was assessed using a dual-luciferase reporter-assay system (Promega, Madison, USA).
Wu W., He Y., Feng X., Ye S., Wang H., Tan W., Yu C., Hu J., Zheng R, & Zhou Y. (2016). MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor. Oncotarget, 8(1), 705-721.
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2

Investigating miR-362-3p Regulation of MCM5

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Targetscan database and miRanda database were used to analyze the putative target genes of miR-362-3p. The MCM5 wild-type (wt) and mutant (mut) 3′-UTR were created and cloned to the firefly luciferase-expressing vector, the pmiR-RB-REPORT luciferase reporter plasmid (RiboBio, China). For the luciferase assay, Hela and GH354 cells were seeded in 24-well plates and cotransfected with the pmiR-MCM5-wt/pmiR-MCM5-mut and mimics-miR-363-3p/inhibitor-miR-363-3p by using lipofectamine 3000 (Invitrogen, U.S.A.). Luciferase activities were determined with the Dual-Luciferase Reporter System (Promega, U.S.A.).
Wang D., Wang H., Li Y, & Li Q. (2018). MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma. Bioscience Reports, 38(3), BSR20180668.
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