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Direct camp eia kit

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The Direct cAMP EIA Kit is a quantitative enzyme immunoassay designed for the direct measurement of cyclic adenosine monophosphate (cAMP) in biological samples.

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EIA kit (41 citations) Direct cAMP colorimetric (EIA) kit (2 citations)

2 protocols using direct camp eia kit

1

Eugenol-Induced cAMP Modulation

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Before treatment with PNF and piperine, the 3T3 L1 cells were starved with serum-free DMEM (high glucose) media for 16–18 h. After 30 min of pretreatment with PNF and piperine, the cells were treated with eugenol for 7 min and then lysed with 0.1 M HCl. cAMP levels were measured using the Direct cAMP EIA Kit (Enzo Life Sciences).
Yoon Y.C., Kim S.H., Kim M.J., Yang H.J., Rhyu M.R, & Park J.H. (2014). Piperine, a component of black pepper, decreases eugenol-induced cAMP and calcium levels in non-chemosensory 3T3-L1 cells. FEBS Open Bio, 5, 20-25.
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2

Bordetella pertussis ACT-induced cAMP Assay

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cAMP produced by ACT-coated bacteria (Bordetella pertussis 18323 H-) (incubation of 5–100 μg ACT/106 bacteria) was assayed for 10 min at 37 °C with 2 nM CaM in AC reaction buffer (30 mM Tris-HCl, pH 7.4, 20 mM MgCl2 and 100 μM CaCl2), then the reaction was started by addition of 5 mM ATP. After 10 min at 37 °C with continuous stirring the reaction was stopped with 0.1 M HCl. The cAMP production was calculated by the direct cAMP EIA kit (Enzo lifesciences).
Martín C., Etxaniz A., Uribe K.B., Etxebarria A., González-Bullón D., Arlucea J., Goñi F.M., Aréchaga J, & Ostolaza H. (2015). Adenylate Cyclase Toxin promotes bacterial internalisation into non phagocytic cells. Scientific Reports, 5, 13774.
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