Cells were lysed in RIPA lysis buffer (BD Pharmingen, San Diego, CA, USA), protein concentration was measured by BCA reagent kit (Beyotime Institute of Biotechnology, Haimen, China). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate cellular lysates, then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Boston, MA, USA). The transfected membranes were blocked in 5% skim milk and incubated in 4°C overnight using the following primary antibodies: anti-ATF1 (1:1,000), anti-ATM (1:1,000), anti-ATM (phospho-1981; 1:5,000) (all from Abcam, Cambridge, MA, USA), anti-p53 (1:800), anti-p21 (1:500), anti-Bax (1:1,000) and anti-Bcl-2 (1:500) (all from Wanleibio, Shenyang, China), anti-GAPDH (1:3,000; Proteintech Group, Inc., Chicago, IL, USA). Then incubated with horseradish peroxidase-conjugated secondary antibody, goat anti-rabbit (1:5,000) or goat anti-mouse (1:5,000) (both from Cell Signaling Technology, Inc., Danvers, MA, USA). All bands were visualized with enhanced chemiluminescence (ECL) kit (Millipore).
Anti p21
Manufactured by Wanlei
Sourced in United States
Anti-P21 is a laboratory reagent used to detect and quantify the presence of the p21 protein, a key regulator of cell cycle progression. It is a critical tool for researchers studying cell biology and cancer-related pathways.
Automatically generated - may contain errors
Lab products found in correlation
Anti p53, by Wanlei (2 mentions)
Anti bax, by Wanlei (2 mentions)
Anti bcl 2, by Wanlei (2 mentions)
Anti pcna, by Wanlei (2 mentions)
Bca reagent kit, by Beyotime (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
Anti atf1, by Abcam (1 mentions)
Ecl kit, by Merck Group (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Anti gapdh, by Proteintech (1 mentions)
Ab226977, by Abcam (1 mentions)
Anti myod, by Abcam (1 mentions)
Anti cdk2, by Wanlei (1 mentions)
Anti cycline, by Wanlei (1 mentions)
Anti insr, by Abcam (1 mentions)
Pvdf membrane, by Thermo Fisher Scientific (1 mentions)
Ripa lysis buffer, by Solarbio (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
Anti cdk2, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
3 protocols using anti p21
1
Western Blot Protein Expression Analysis
2
Protein Expression Profiling in Bovine Myoblasts
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Total proteins of bovine myoblasts were prepared with radio immunoprecipitation assay (RIPA) lysis buffer (Solarbio, Beijing, China). Proteins were fractionated on 12% SDS-PAGE gels and transferred to polyvinylidene difluoride (PVDF) membranes (Thermo Fisher Scientific). Blots were incubated overnight with primary antibodies specific for anti-CyclinD1 (#ab226977), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, #ab9485), anti-MyoD (#ab16148) (Abcam, Cambridge, UK), anti-INSR (#WL02857), anti-PCNA (#WL01804), anti-CDK2 (#WL01543), anti-CyclinE (#WL01072), anti-Bcl-2 (#WL01556), anti-P53 (#WL01919), anti-P21 (#WL0362), anti-MyoG (#WL01132), anti-MyHC (#WL02785), and anti-Bax (#WL01637) (Wanleibio, Haerbin, China) at 4°C. After incubation with secondary antibodies, the membranes were quantified with the ChemiDoc XRS system (Bio-Rad, Hercules, CA, USA).
3
Curcumol Inhibits Cancer Cell Growth
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Curcumol (purity > 98%) was purchased from Guizhou Dida Technology Co., Ltd (Guizhou, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) were purchased from Amresco, LLC (Solon, OH, USA). DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Anti-rabbit IgG, anti-mouse IgG and antibodies against β-actin were purchased from ZSGB-BIO (Beijing, China). The anti-PI3 Kinase P110 β, anti-PI3 Kinase P85 α, anti-Nucleolin, anti-AKT, anti-phosphor(p)-AKT, anti-PARP-1, anti-P53, anti-Bcl-2 were purchased from Abcam (Cambridge, MA, USA), anti-CDK6, anti-CyclinD1, anti-CDK2 were purchased from Cell Signaling Technology (Danvers, MA, USA), anti-PCNA, anti-P21 were purchased from Wanleibio, LLC (Shenyang, China), anti-ERα36 antibody and plasmid vector were provided by Zhao Yi Wang (Creighton University Medical School).
Anti-rabbit IgG, anti-mouse IgG and antibodies against β-actin were purchased from ZSGB-BIO (Beijing, China). The anti-PI3 Kinase P110 β, anti-PI3 Kinase P85 α, anti-Nucleolin, anti-AKT, anti-phosphor(p)-AKT, anti-PARP-1, anti-P53, anti-Bcl-2 were purchased from Abcam (Cambridge, MA, USA), anti-CDK6, anti-CyclinD1, anti-CDK2 were purchased from Cell Signaling Technology (Danvers, MA, USA), anti-PCNA, anti-P21 were purchased from Wanleibio, LLC (Shenyang, China), anti-ERα36 antibody and plasmid vector were provided by Zhao Yi Wang (Creighton University Medical School).
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