Peritoneal exudate cells (PECs) were collected by lavage with 10 mL of flow buffer (1% FBS in PBS (Corning)). PECs were washed once, then Fc receptors were blocked with α-CD16/32 (Biolegend). The following antibodies were used in various experiments: Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend), APC-Cy7-α-CD19 (1D3, BD Biosciences), redFluor 710-α-CD11b (M1/70, Tonbo), Fitc-α-CD11b (M1/70, BD Biosciences), PE-Cy7-α-CD19 (1D3, Tonbo), Violetfluor450-α-CD3 (17A2, Tonbo), and BV711-α-Siglec F (E50-2440, BD Biosciences). Cells were fixed with 2% formaldehyde (VWR). Samples were run on a Novoctye 3000 (ACEA Biosciences) and analyzed with either Flowjo (Tree Star, Inc, San Carols, CA) or NovoExpress (version 1.5.0, Agilent Technologies, Inc.).
Violetfluor450 α cd3
Manufactured by Cytek Biosciences
Violetfluor450-α-CD3 is a fluorochrome-conjugated antibody that specifically binds to the CD3 antigen on T cells. It is designed for use in flow cytometry applications to identify and quantify T cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Formaldehyde, by Avantor (2 mentions)
αcd16 32, by BioLegend (2 mentions)
Apc cy7 α cd19, by BD (2 mentions)
Alexafluor 647 α cd102 3c4, by BioLegend (2 mentions)
Redfluor 710 α cd11b, by Cytek Biosciences (2 mentions)
Fitc α cd11b, by BD (2 mentions)
Pe cy7 α cd19, by Cytek Biosciences (2 mentions)
Bv711 α siglec f, by BD (2 mentions)
Novoctye 3000, by Agilent Technologies (2 mentions)
Novoexpress, by Agilent Technologies (2 mentions)
Phosphate buffered saline (pbs), by Corning (2 mentions)
2 protocols using violetfluor450 α cd3
1
Isolation and Immunophenotyping of Peritoneal Exudate Cells
2
Isolation and Immunophenotyping of Peritoneal Exudate Cells
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Peritoneal exudate cells (PECs) were collected by lavage with 10 mL of flow buffer (1% FBS in PBS (Corning)). PECs were washed once, then Fc receptors were blocked with α-CD16/32 (Biolegend). The following antibodies were used in various experiments: Alexafluor 647-α-CD102 (3C4 (MIC2/4), Biolegend), APC-Cy7-α-CD19 (1D3, BD Biosciences), redFluor 710-α-CD11b (M1/70, Tonbo), Fitc-α-CD11b (M1/70, BD Biosciences), PE-Cy7-α-CD19 (1D3, Tonbo), Violetfluor450-α-CD3 (17A2, Tonbo), and BV711-α-Siglec F (E50-2440, BD Biosciences). Cells were fixed with 2% formaldehyde (VWR). Samples were run on a Novoctye 3000 (ACEA Biosciences) and analyzed with either Flowjo (Tree Star, Inc, San Carols, CA) or NovoExpress (version 1.5.0, Agilent Technologies, Inc.).
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