Single‐cell fertilized eggs were obtained by either natural or induced breeding and injected according to previously published protocols with modifications.
Mmessage t7 ultra transcription kit
The MMESSAGE T7 ULTRA Transcription Kit is a laboratory product designed for in vitro transcription of RNA. It provides a simple and efficient method for generating high yields of RNA from DNA templates.
Lab products found in correlation
5 protocols using mmessage t7 ultra transcription kit
CRISPR-Cas9-Mediated Genome Editing in Newts
Single‐cell fertilized eggs were obtained by either natural or induced breeding and injected according to previously published protocols with modifications.
CRISPR-Cas9 Mediated Genome Editing in Newts
Single-cell fertilized eggs were obtained by either natural or induced breeding and injected according to previously published protocols with modifications60 (link), 61 (link). Briefly, 500 pg Cas9 RNA and 100 pg gRNA were mixed into 5 nl and injected into freshly laid single-cell-stage embryos. The animals were raised according to60 (link). The screening of F0 animals was done by both genotyping (see above) and phenotype characterization, including pigmentation analysis and immunohistochemistry of limbs/tails, according to39 (link), 40 (link). The Pax7−/− and Tyr−/− F1 animals were produced by crossing between adult F0 animals. The larvae or post-metamorphic newts were anaesthetized within 0.01% or 0.1% ethyl-p-aminobenzoate (benzocaine; Sigma) prior to imaging and tissue collection. Newts utilized for this study were processed according to Swedish Board of Agriculture animal ethical regulations (N429/12).
Expanded Papilloma T Cell Reactivity
Cloning and Mutagenesis of spHCN Channel
Engineered spHCN channel for ACCuRET experiments
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