IHC staining and evaluation of the IHC analysis on CRC tissue microarray samples was performed as previously described [8 (link)]. The primary antibody include anti-TNC (1:100, Abcam, UK), anti-SOX2 (1:100, Abcam, UK), anti-CD44 (1:80, Millipore, USA), anti-CD133 (1:100, NOVUS, USA), anti-LSD1 (1:250, Sigma, USA), anti-SOX9 (1:100, Abnova, USA), anti-p21 (1:100, Millipore, USA), anti-cyclinD1 (1:100, Millipore, USA), anti-p27 (1:100, Millipore, USA), anti-CDK4 (1:100, Millipore, USA), anti-p16 (1:100, Millipore, USA), anti-SMO (1:250, Santa, USA), and anti-GLI1 (1:100, Abcam, UK).
Anti p16
Manufactured by Merck Group
Sourced in United States
Anti-p16 is a laboratory reagent used for the detection and analysis of the p16 protein, which is a tumor suppressor and cell cycle regulator. It is commonly used in research applications to study the role of p16 in various cellular processes and disease states.
Automatically generated - may contain errors
Lab products found in correlation
Anti cyclin d1, by Merck Group (1 mentions)
Anti p21, by Merck Group (1 mentions)
Anti sox2, by Abcam (1 mentions)
Anti cd44, by Merck Group (1 mentions)
Anti lsd1, by Merck Group (1 mentions)
Anti gli1, by Abcam (1 mentions)
Anti cd133, by Novus Biologicals (1 mentions)
Anti tnc, by Abcam (1 mentions)
Anti sox9, by Abnova (1 mentions)
Anti β catenin, by Thermo Fisher Scientific (1 mentions)
Anti p rb, by Abcam (1 mentions)
2 protocols using anti p16
1
Immunohistochemical Profiling of CRC Samples
2
Immunoblotting for Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunoblotting was performed as described elsewhere. The following primary antibodies were used: anti‐β‐catenin (Invitrogen, 15B8, 1:1000), anti‐pERK1/2 MAPK (Cell Signaling, 1:2000), anti‐pospho‐pERK1/2MAPK (Cell Signaling, 1:2000), anti‐pRb (Abcam, 1:1000), and anti‐p16 (Millipore, 1:1000). To verify equal loading of samples, the membranes were incubated with monoclonal β‐actin antibody, followed by an HRP‐conjugated goat anti‐mouse IgG.
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