Hamilton syringe 26 g

All animal studies were performed according to the protocols approved by the Institutional Animal Care and Use Committee, in accordance with the NIH and USDA guidelines. Intracerebral hemorrhage was induced in aged male C57BL/6 mice (18–24 months), (Jackson Laboratories, Bar Harbor, ME, USA), as previously reported by our laboratory [2 (link),30 (link),31 (link),32 (link),33 (link)]. Briefly, mice were anesthetized with isoflurane and positioned prone on a stereotaxic head frame (Stoelting, Wood Dale, IL, USA). Using a high-speed drill (Dremel, Racine, WI, USA), a burr hole (0.5 mm) was made 2.2 mm lateral to the bregma, and a small animal temperature controller (David Kopf Instruments, Los Angeles, CA, USA) was used to keep the body temperature at 37 ± 0.5 °C. Employing a Hamilton syringe (26-G), 0.04 U of bacterial type IV collagenase (Sigma, St. Louis, MO, USA) in 0.5 μL phosphate-buffered saline (phosphate buffered saline; pH 7.4 (PBS) was injected with the stereotaxic guidance 3.0 mm into the left striatum to induce ICH [2 (link)]. After removing the needle, bone wax was used to seal the burr hole and the incision was stapled. Sham mice underwent the same surgical procedure, but only PBS (0.5 μL) was injected, which served as the experimental control.

Animal studies were reviewed and approved by the Committee on Animal Use for Research and Education at Augusta University, in compliance with NIH and USDA guidelines. ICH was induced in adult male CD-1 mice (Charles River) as previously reported (Sukumari-Ramesh et al., 2012a (link),b (link), 2016 (link); Bonsack et al., 2016 (link); Sukumari-Ramesh and Alleyne, 2016 (link)). Briefly, mice (n = 72) were anesthetized with ketamine and xylazine and prone-positioned on a stereotaxic head frame (Stoelting, WI, U.S.A.). The body temperature was maintained at 37 ± 0.5°C during the surgical procedure using a small animal temperature controller (David Kopf Instruments, USA) and a burr hole (0.5 mm) was made 2.2 mm lateral to bregma using a high-speed drill (Dremel, USA) without damaging the underlying dura. A Hamilton syringe (26-G) containing 0.04U of bacterial type IV collagenase (Sigma, St. Louis, MO) in 0.5 μL phosphate buffered saline (pH 7.4; PBS) was inserted with stereotaxic guidance 3.0 mm into the left striatum to induce spontaneous ICH (Bonsack et al., 2016 (link)). After removal of the needle, the burr hole was sealed with bone wax and the incision was stapled. Sham mice underwent the same surgical procedure, but only PBS (0.5 μL) was injected.