DMEM complete culture medium, MTT assay (Shanghai Jingke Chemistry Technology Co., Ltd); Fetal bovine serum, 0.25% trypsin digestive solution, 0.08% trypsin digestive solution (Gibco); Dimethyl sulfoxide (DMSO) cell freezing medium (Sigma); 0.1mmol/L phosphate buffer (PBS) (Central Laboratory of Xiangyang Hospital Affiliated to Hubei University of Medicine); Copper-containing stainless steel material leach liquor and ordinary stainless steel material leach liquor (Institute of Metal Research, Chinese Academy of Science); Rabbit anti-rat Fas/FasL polyclonal antibody, Anti-GAPDH antibody, standard protein marker (Abcom); Agarose and polyvinylidene fluoride (PVDF) membrane (Beijing North TZ-Biotech Develop, Co. Ltd.); BAC protein concentration measuring kit and ECL kit (Beijing Dingguo Changsheng Biotechnology Co., Ltd.); II antibody, Ethylene Diamine Tetraacetic Acid (EDTA), Sodium dodecyl sulfate (SDS), N’-Tetramethylethylenediamine (TEMED), Tris(hydroxymethyl)methyl aminomethane THAM (Tris) (Sigma); Various gel preparation solutions and buffers (Central Laboratory of Jinzhou Medical University).
TUNEL apoptosis in situ detection kit was provided by Boster Biological Technology Co. Ltd, Wuhan, China.
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