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β naphthoflavone β nf

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β-naphthoflavone (β-NF) is a laboratory compound used in research applications. It is a flavonoid derivative with a specific chemical structure. β-NF can be utilized as a tool compound in various scientific investigations, but its core function is not to be interpreted or extrapolated beyond a factual description.

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Lab products found in correlation

Chlorzoxazone, by Merck Group (2 mentions) Tolbutamide, by Merck Group (2 mentions) Bupropion, by Merck Group (2 mentions) Acetaminophen, by Merck Group (2 mentions) Caffeine, by Merck Group (2 mentions) Nicotine, by Merck Group (2 mentions) Warfarin, by Merck Group (2 mentions) Hplc grade acetonitrile, by Merck Group (2 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) α naphthoflavone α nf, by Merck Group (1 mentions) Antibiotic antimycotic, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Metoprolol, by Merck Group (1 mentions) Carvedilol, by Merck Group (1 mentions) Adenosine 3 phosphate 5 phosphosulfate lithium salt hydrate, by Merck Group (1 mentions) Caffeine 13c3, by Merck Group (1 mentions) Glutathione (gsh), by Merck Group (1 mentions) Elagic acid, by Merck Group (1 mentions) 2 4 dinitrophenol, by Merck Group (1 mentions) Triton x 100, by Thermo Fisher Scientific (1 mentions)

4 protocols using β naphthoflavone β nf

1

HL-60 Cell Culture and Treatments

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HL-60 human myeloblastic leukemia cells derived from the original patient isolate, a generous gift of Dr. Robert Gallagher, were grown in RPMI 1640 (Invitrogen, Carlsbad, CA) supplemented with 5% fetal bovine serum (Hyclone, Logan,UT) and 1x antibiotic/antimycotic (Sigma, St. Louis, MO) in a 5% CO2 humidified atmosphere at 37°C. The cells were cultured in constant exponential growth as previously described [54 (link)]. The experimental cultures were initiated at a density of 0.1 x 106 cells/ml. Viability was monitored by 0.2% trypan blue (Invitrogen, Calsbad, CA) exclusion and routinely exceeded 95%.
For treatments, all-trans-retinoic acid (RA) (Sigma, St. Louis, MO) was added from a 5 mM stock solution in 100% ethanol to a final concentration of 1 μM in culture. 6-Formylindolo(3,2-b)carbazole (FICZ) (BML-GR206-0100, Enzo Life Sciences, Exeter, United Kingdom and Abcam, Cambridge, MA ab141631), was added from a 100 μM DMSO stock to make a final concentration of 100 nM in culture. α-naphthoflavone (α-NF)and β-naphthoflavone (β-NF, both from Sigma, St. Louis, MO) were each used at a final concentration of 1 μM in culture.
Bunaciu R.P., Jensen H.A., MacDonald R.J., LaTocha D.H., Varner J.D, & Yen A. (2015). 6-Formylindolo(3,2-b)Carbazole (FICZ) Modulates the Signalsome Responsible for RA-Induced Differentiation of HL-60 Myeloblastic Leukemia Cells. PLoS ONE, 10(8), e0135668.
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2

Comprehensive Metabolic Profiling Assay

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Acetaminophen (APAP) (>99%), β-naphthoflavone (β-NF) (>98%), caffeine (>99%), 13C3-caffeine (>99%), nicotine (>99%), chlorzoxazone (CLZ) (98%), tolbutamide (>99%), bupropion (98%), warfarin (>98%), carvedilol (>98%), metoprolol (>98%), adenosine 3′-phosphate 5′-phosphosulfate lithium salt hydrate (PAPS) (>60%), glutathione (GSH) (98%), acetyl-coenzyme A (acetyl-CoA) (93%), and HPLC grade acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO). caffeine-d3 (1-methyl-d3) (99%) and bupropion-d9 (tert-butyl-d9) (99%) were purchased from CDN isotopes (Point-Clare, Quebec, Canada). Pazopanib, benzo[a]pyrene, and uridine diphosphate glucuronic acid (UDPGA) (>98%) were purchased from US Biological (Salem, MA). Other precursors (>95% purity) were sourced from the Spectrum Collection purchased from Microsource Discovery Systems (Gaylordsville, CT). Pooled human liver S9 fraction: mixed-gender (from 50 livers, H0640.S9) and NADPH regenerating system (K5000-10) were from Sekisui Xenotech (Kansas City, KS).
Liu K.H., Lee C.M., Singer G., Bais P., Castellanos F., Woodworth M.H., Ziegler T.R., Kraft C.S., Miller G.W., Li S., Go Y.M., Morgan E.T, & Jones D.P. (2021). Large scale enzyme based xenobiotic identification for exposomics. Nature Communications, 12, 5418.
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3

Bioassay Protocol for Yeast Strains

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2.1 Reagents, microorganisms and cell lines 2,4-dinitrophenol, tannic acid, elagic acid, gallic acid, protecatechuic acid, vanillic acid, syringic acid and sinapic acid were purchased from Sigma-Aldrich and used as received. Ultra-pure water ( Mili-Q) was used to prepare all solutions. P. subcapitata and ephippia (dormant eggs) of D. magna, employed for the bioassays were supplied by ECOtest S.L. (Valencia, Spain) . Two recombinant strains of Saccharomyces cerevisiae were employed: BY4741 strain to detect estrogenic activity (ER strain) and YCM4 strain to monitor aryl hydrocarbon receptor agonist activity (AhR strain); they were kindly provided by Dr. Benjamin Piña from IDAEA-CSIC, Barcelona, Spain.
Medium constituents employed in bioassays were supplied by Difco (Basel, Switzerland). Prototrophic markers, standards (17-β-estradiol (E2) and βnaphthoflavone (β-NF)) and enzymatic substrate (4-methylumbelliferone β-Dgalactopyranoside and β-mercaptoethanol) were purchased form Sigma-Aldrich. Assay buffer (Triton X-100) and Y-PER (Yeast Protein Extraction Reagent) were supplied by Fisher Chemical and Thermo Scientific, respectively.
García-Ballesteros S., Mora M., Vicente R., Vercher R.F., Sabater C., Castillo M.A., Amat A.M, & Arques A. (2019). A new methodology to assess the performance of AOPs in complex samples: Application to the degradation of phenolic compounds by O3 and O3/UV-A-Vis. Chemosphere, 222.
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4

Compound Acquisition and Antibody Procurement

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Acenaphthene, acetaminophen, auranofin, β-naphthoflavone (β-NF), benzo[a]pyrene, bupropion, caffeine, chlorzoxazone (CLZ), nicotine, tolbutamide, trifluralin, naphthalene, coumarin, fluoranthene, 1,2-diphenylhydrazine, ethylbenzene, 2,4,6-trichlorophenol, estrone, 1,1,2,2-tetrachloroethane, N-nitrosodipropylamine, 2-methylnaphthalene, endrin aldehyde, azinphos-methyl, dexamethasone, Geneticin (G418), pazopanib, S,S,S tributyl-phosphorotrithioate, bromodichloromethane, ethion, benzo[b]fluoranthene, bis(2-ethylhexyl)phthalate, crizotinib, 1,2-dichlorobenzene, o,p'dichlorodiphenyldichloroethane (DDD), omeprazole, pazopanib, polybrene, warfarin and HPLC grade acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO). S,Shydroxy and R,R-hydroxy bupropion were purchased from Cayman Chemical, Ann Arbor, MI) and vandetanib was from AstaTech (Bristol, PA). Blasticidin was obtained from InvivoGen (San Diego, CA). Rabbit anti-V5 (V8137), mouse anti-glyceraldehyde 6phosphate dehydrogenase (MAB374), and mouse anti-actin (A2228) antibodies were purchased from Sigma.
Lee C., Liu K., Singer G., Miller G.W., Li S., Jones D.P., & Morgan E.T. (2022). High-throughput Production of Diverse Xenobiotic Metabolites with P450-transduced Huh7 Hepatoma Cell Lines.
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