Adult DRG were dissected and collected in Hank’s Balanced Salt Solution (HBSS) on ice and digested according to standard procedures. Cultures were maintained in medium containing B27 and penicillin–streptomycin in DMEM:F12. Cells were plated on coated glass coverslips (0.01 mg ml−1PDL) for 48-72 h and fixed with 4% PFA–4% sucrose. Immunocytochemistry was performed by incubating fixed cells with Cdkl5 (Sigma HPA002847), βIII-tubulin (Abcam ab18207), CaMKIIα (Thermo-Fisher MA1-048), (Abcam ab81887), Parvalbumin (Abcam ab555), GFP (Thermo-Fisher A-6455), HA (Abcam ab18181) antibodies at 4 °C overnight. This was followed by incubation with AlexaFluor-conjugated goat secondary antibodies according to standard protocols (Invitrogen). All cells were counterstained with Hoechst (Molecular Probes).
Cdkl5
Manufactured by Merck Group
Sourced in United States
Cdkl5 is a lab equipment product manufactured by Merck Group. It is a protein kinase that plays a role in neurological processes. The core function of Cdkl5 is to phosphorylate various substrates, which is a crucial step in cellular signaling pathways. Further details on the intended use of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst, by Thermo Fisher Scientific (1 mentions)
Parvalbumin, by Abcam (1 mentions)
βiii tubulin, by Abcam (1 mentions)
Camkiiα, by Thermo Fisher Scientific (1 mentions)
Alexa fluor conjugated goat secondary antibodies, by Thermo Fisher Scientific (1 mentions)
α tubulin, by Merck Group (1 mentions)
Phospho h3s10, by Abcam (1 mentions)
Centrin 2, by Santa Cruz Biotechnology (1 mentions)
Mklp1, by Santa Cruz Biotechnology (1 mentions)
Cep170, by Thermo Fisher Scientific (1 mentions)
β tubulin, by Merck Group (1 mentions)
β actin, by Merck Group (1 mentions)
γ tubulin, by Merck Group (1 mentions)
2 protocols using cdkl5
1
Immunocytochemical Analysis of DRG Neurons
2
Antibody Detection Protocol for Cellular Proteins
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Antibodies (Abs) against the following proteins were used: β-actin (Sigma-Aldrich, St Louis, MO, USA, A5441), α-tubulin (Sigma-Aldrich, T6074), β-tubulin (Sigma-Aldrich, C4585), γ-tubulin (Sigma-Aldrich, T5326), CDKL5 (polyclonal, Sigma-Aldrich, HPA002847; monoclonal Santa Cruz, clone D-12, sc-376314), AKT (Cell Signaling, 4685), Aurora kinase A (Sigma-Aldrich, A1231, clone 35C1), GFP (Roche Diagnostics Ltd, 1814460), PLK-1 (Santa Cruz, sc-17783), phospho-H3-S10 (Abcam, ab14955), H2SB14-P (Cell Signaling, 6959); centrin-2 (Santa Cruz, sc-27793), Cep170 (ThermoFisher Scientific, 41-3200) and HIPK2 (rabbit polyclonal21 (link)); MgcRacGAP1 (Abcam, ab2270), MKLP1 (Santa Cruz; sc-22793); PRC1 (Santa Cruz, Sc-8356); spastin (Santa Cruz, Sc-53443), CREST centromere protein (Antibodies Inc., 15-234-0001). HRP-conjugated goat anti-mouse or anti-rabbit secondary Abs for immunoblotting were purchased from Thermo Fisher Scientific. DAPI and secondary Alexa Fluor anti-rabbit, anti-mouse, anti-human, and anti-goat Abs for immunofluorescence were obtained from Life Technologies while FITC- or TRITC-conjugated Abs were from Jackson Immuno Research Lab.
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