Anti gapdh

Manufactured by ProSci

Anti-GAPDH is a laboratory reagent used in various experimental techniques to detect and quantify the presence of the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein. GAPDH is a widely used reference or housekeeping protein for data normalization in biological research.

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Lab products found in correlation

Sab4200181, by Merck Group (1 mentions) Hpa012145, by Merck Group (1 mentions) Ab179843, by Abcam (1 mentions) Adenosine triphosphate (atp), by Sangon (1 mentions) Pyruvate kinase, by Merck Group (1 mentions) Z vad fmk, by WuXi AppTec (1 mentions) Gsk872 hy 101872, by MedChemExpress (1 mentions) V5 beads, by Merck Group (1 mentions) Flag bead, by Merck Group (1 mentions) Nec 1, by Merck Group (1 mentions) Nec 1, by Abcam (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) Anti β actin, by ABclonal (1 mentions) Anti myc hrp, by Cell Signaling Technology (1 mentions)

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GAPDH (5 citations)

2 protocols using anti gapdh

Antibodies for ACBD5, PEX14, VAPB Immunoblotting

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PLA: Anti-ACBD5 rabbit antibody (HPA012145; Sigma-Aldrich), anti-PEX14 rabbit antibody (Grant et al., 2013 (link)) (generated by D. Crane, Griffith University, Brisbane, Australia) and anti-VAPB mouse antibody (66191-1-Ig; Proteintech) were used. Immunoblots: Anti-ACBD5, PEX14 and VAPB as above, Anti PMP70 (SAB4200181; Sigma) Anti-PEX11β (ab 182100; Abcam), Anti-Catalase (ab179843; Abcam), Anti-GAPDH (ProSci).

Bishop A., Kamoshita M., Passmore J.B., Hacker C., Schrader T.A., Waterham H.R., Costello J.L, & Schrader M. (2019). Fluorescent tools to analyse peroxisome-ER interactions in mammalian cells. Contact (Thousand Oaks (Ventura County, Calif.)), 2, 10.1177/2515256419848641.

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Immunoblotting Antibody Reagents and Protocols

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For immunoblotting, antibodies were purchased from Sigma-Aldrich (HRP-anti-Flag, Cat# A8592; anti-Myc-HRP, Cat# 16-213), Cell Signaling Technology (HRP-anti-rabbit IgG, Cat# 7074P2; HRP-anti-mouse IgG, Cat# 7076; anti-β-actin, Cat# 4967; anti-RIP1, Cat# 3493; anti-capsase8, Cat# 9746), ABclonal (anti-GAPDH, Cat# AC033; anti-HSPA8, Cat# A10898), Prosci (anti-mouse RIP3, Cat# 2283) and Abcam (anti-LAMP2A, ab125068; anti-V5-HRP, ab1325; anti-6×His, ab18184; anti-human p-MLKL, ab187091; anti-mouse p-MLKL, ab196436). Anti-human MLKL, anti-mouse MLKL and anti-human RIP3 antibodies are generated by our own laboratory.
Recombinant TNF was purified in the lab. ATP (#A600020-0005) was purchased from Sangon. Smac mimetics LCL161 (HY-15518), GSK872 (HY-101872), and NSA (HY-100573) were purchased from MedChemExpress. Trypsin (#25200072) was purchased from ThermoFisher Scientific. ThT (#596200), Flag beads (#M8823), Myc beads (#E6654), V5 beads (#A7345), Nec-1 (#N9037) phosphoenolpyruvate (#P7127) and pyruvate kinase (#P7768) were purchased from Sigma-Aldrich. Nec-1s (#2263) was purchased from BioVision. The TRIF_(677–698) proteins and Z-VAD-FMK were custom-made by WuXi AppTec. Recombinant RHIM-containing proteins (RIP1_(498–582), RIP3_(418–518), and ZBP1_(150–293)) and recombinant α-syn were generated previously.

Wu E., He W., Wu C., Chen Z., Zhou S., Wu X., Hu Z., Jia K., Pan J., Wang L., Qin J., Liu D., Lu J., Wang H., Li J., Wang S, & Sun L. (2023). HSPA8 acts as an amyloidase to suppress necroptosis by inhibiting and reversing functional amyloid formation. Cell Research, 33(11), 851-866.

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