Immunohistochemistry was performed on archival formalin-fixed paraffin-embedded duodenal sections of 4 patients with active CD and 4 controls. The sections were deparaffinized and dehydrated through xylene and ethanol and the antigen retrieval was performed in citrate buffer (pH 6.0) for 20 min in a microwave. Immunohistochemical staining was performed using a mouse monoclonal antibody directed against human AhR (ab2770, 1:150 final dilution; Abcam, Cambridge, MA, USA) at room temperature for 1 h followed by a biotin-free HRP-polymer detection technology with 3,3'diaminobenzidine (DAB) as a chromogen (UltraVision kit, Lab Vision, Fremont, CA, USA). The sections were counterstained with haematoxylin, dehydrated, and mounted. Isotype control IgG-stained sections were prepared under identical immunohistochemical conditions as described above, replacing the primary AhR antibody with a purified control isotype (R&D Systems).
Ultravision kit
Manufactured by Lab Vision
Sourced in United States
The UltraVision kit is a laboratory tool designed for visual inspection and analysis. It provides high-resolution imaging capabilities to support various scientific and research applications.
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Lab products found in correlation
2 protocols using ultravision kit
1
Duodenal AhR Expression in Celiac Disease
2
Immunohistochemical Analysis of PAD4 in Ulcerative Colitis
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Immunohistochemistry was performed on archival formalin-fixed paraffin-embedded colonic sections of five patients with UC and five normal controls [NC]. Additional sections were prepared from samples taken from the inflamed and non-inflamed areas of four patients with UC. Colonic sections of three patients with UC before and after IFX treatment were also analysed. The sections were deparaffinized and dehydrated through xylene and ethanol and antigen retrieval was performed in citrate buffer [pH 6.0] for 20 min in a microwave. Immunohistochemical staining was performed using a mouse monoclonal antibody directed against human PAD4 [1:5000 final dilution; Novus Biologicals] at room temperature for 1 h followed by a biotin-free horseradish peroxidase [HRP]-polymer detection technology with 3,3′diaminobenzidine [DAB] as a chromogen [UltraVision kit, Lab Vision]. The sections were counterstained with haematoxylin, dehydrated and mounted. Isotype control IgG-stained sections were prepared under identical immunohistochemical conditions as described above, replacing the primary PAD4 antibody with a purified control isotype [R&D Systems].
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