Commercially available kits were used to measure cytokine levels of INF-λ, sIL-6R (ELISA, R&D Systems, USA), IL-6, IL-1β, IFN-γ (ELISA, Biolegend, USA), and TNF-α (CBA, BD Biosciences, USA) in BALF or lung homogenate. PGD2 was measured in lung homogenates using a PGD2-MOX Express ELISA kit (Cayman Chemicals, Michigan, USA).
Pgd2 mox express elisa kit
Manufactured by Cayman Chemical
Sourced in United States
The PGD2-MOX Express ELISA kit is a quantitative in vitro diagnostic test used to measure the concentration of 15-Deoxy-Delta-12,14-PGJ2 in biological samples. The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique to detect the target analyte.
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Lab products found in correlation
2 protocols using pgd2 mox express elisa kit
1
Cytokine and Eicosanoid Quantification
2
PGD2 Quantification in Biological Samples
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Briefly, lung lobes were homogenized, or supernatant was collected and then liquid-extracted using acetone. Samples were spun and washed twice and then evaporated to dryness under a stream of compressed air (about 2 min). All samples (lung samples, nasal swabs, and cell supernatant) were methyloximated to stabilize PGD2, and the assay was run as per the manufacturer's instructions. A PGD2-MOX Express ELISA kit (mouse) or a PGD2-MOX ELISA kit (human samples) was used to detect PGD2 production (Cayman Chemical). According to the manufacturer, cross-reactivity with similar molecules (for example, PGF2 or prostaglandin E2) is <1%.
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