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The B5002 is a laboratory centrifuge designed for general-purpose sample separation. It is capable of generating centrifugal forces up to 4,000 x g. The B5002 is equipped with a brushless motor and digital speed control for precise speed regulation.

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Lab products found in correlation

N3633, by Merck Group (2 mentions) C10337, by Thermo Fisher Scientific (2 mentions) C8267, by Merck Group (2 mentions)

3 protocols using b5002

1

Induction of Cre Expression and DNA Synthesis Detection

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To induce Ah-cre expression, 80mg kg−1 body weight of β-naphthoflavone (Sigma-Aldrich; N3633) dissolved in corn oil (Sigma-Aldrich; C8267) was administrated into 2-month old mice with 5 injections within 30 hours. For DNA synthesis detection, 100mg kg−1 body weight of BrdU (Sigma-Aldrich; B5002) or 5mg kg−1 body weight of EdU (Life Technologies; C10337) dissolved in sterile phosphate-buffered saline (PBS) was intraperitoneally injected 2 hours or 1 hour before the mice were sacrificed, respectively. EdU staining was performed following manufacturer’s protocol (Life Technologies; C10337).
Liu H., Tang X., Srivastava A., Pécot T., Daniel P., Hemmelgarn B., Reyes S., Fackler N., Bajwa A., Kladney R., Koivisto C., Chen Z., Wang Q., Huang K., Machiraju R., Sáenz-Robles M.T., Cantalupo P., Pipas J.M, & Leone G. (2015). Redeployment of Myc and E2f1-3 drives Rb deficient cell cycles. Nature cell biology, 17(8), 1036-1048.
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2

Induction of Cre Expression and DNA Synthesis Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce Ah-cre expression, 80mg kg−1 body weight of β-naphthoflavone (Sigma-Aldrich; N3633) dissolved in corn oil (Sigma-Aldrich; C8267) was administrated into 2-month old mice with 5 injections within 30 hours. For DNA synthesis detection, 100mg kg−1 body weight of BrdU (Sigma-Aldrich; B5002) or 5mg kg−1 body weight of EdU (Life Technologies; C10337) dissolved in sterile phosphate-buffered saline (PBS) was intraperitoneally injected 2 hours or 1 hour before the mice were sacrificed, respectively. EdU staining was performed following manufacturer’s protocol (Life Technologies; C10337).
Liu H., Tang X., Srivastava A., Pécot T., Daniel P., Hemmelgarn B., Reyes S., Fackler N., Bajwa A., Kladney R., Koivisto C., Chen Z., Wang Q., Huang K., Machiraju R., Sáenz-Robles M.T., Cantalupo P., Pipas J.M, & Leone G. (2015). Redeployment of Myc and E2f1-3 drives Rb deficient cell cycles. Nature cell biology, 17(8), 1036-1048.
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3

Tracking Cell Cycle and Lineage

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To identify cells that participate in the cell cycle, injections of BrdU (B5002, Life Technologies, 25 mg/kg, ip) were administered every 12 h for 9 days from the time of surgery. To identify LRCs, BrdU (50 mg/kg ip) was injected into pregnant females every 24 h, for a total of two injections, 2–4 days prior to parturition. The newborn mice were healthy, and mice with the CreLacZ phenotype were used in the analysis.
Kanda M., Nagai T., Takahashi T., Liu M.L., Kondou N., Naito A.T., Akazawa H., Sashida G., Iwama A., Komuro I, & Kobayashi Y. (2016). Leukemia Inhibitory Factor Enhances Endogenous Cardiomyocyte Regeneration after Myocardial Infarction. PLoS ONE, 11(5), e0156562.
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