Ho 244966

The purge and trap method was used for analyzing volatile compounds. The headspace mVOCs were absorbed by Tenax-coated columns (PerkinElmer cat #HO244966) made of stainless steel. Totally, four sets, including S. rochei (1 ml of 72-h-old spore suspension) with grain mold pathogens (8 mm 5-day-old mycelia disk), were inoculated in potato broth (PD broth): S. rochei alone, F. moniliforme or C. lunata alone, and both upon tripartite interaction (S. rochei vs. F. moniliforme and C. lunata). Uninoculated PD broth was used as a pessimistic control for the headspace samples. To avoid the dispersal of volatile compounds from the columns, they were sealed with parafilm after the sterilized rubber cork was inserted. The experiment was repeated three times; then, the mycelium was allowed to grow at 28 ± 2°C for 7 days.
The biochemicals produced in the samples were identified using GC-MS, thermal desorber (TD). The resultant mVOCs were compared with NIST 14 standards (Mass Spectral Library). Volatile compounds with a mass spectral resemblance of more than 90% to those in the National Institute of Standards and Technology (NIST) library were categorized as putative active compounds.