Alkaline phosphatase alp

Manufactured by Randox

Sourced in United Kingdom

Alkaline phosphatase (ALP) is an enzyme found in various tissues, including the liver, bone, and kidneys. It plays a crucial role in the hydrolysis of phosphate esters, which is important for various physiological processes. The measurement of ALP levels is a commonly used diagnostic tool to assess liver and bone function.

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Lab products found in correlation

Cobas fara centrifugal analyzer, by Roche (1 mentions) Aspartate aminotransferase, by Randox (1 mentions) Nanophotometer p class, by Implen (1 mentions)

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Alkaline phosphatase

2 protocols using alkaline phosphatase alp

Serum Biochemistry Analysis of Mice

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Blood was collected via cardiac puncture after confirming mouse death and centrifuged at 6000xg 10 min at 4°C. Serum analysis used commercial kits according to the manufacturer’s instructions: serum albumin (Alb), aspartate aminotransferase (AST) and alkaline phosphatase(ALP) (Randox laboratories), Alanine transaminase (ALT), total bilirubin and Amylase (Alpha Laboratories). All kits were adapted for use on a Cobas FARA centrifugal analyzer (Roche Diagnostics).

Hallett J.M., Ferreira-Gonzalez S., Man T.Y., Kilpatrick A.M., Esser H., Thirlwell K., Macmillan M.T., Rodrigo-Torres D., Dwyer B.J., Gadd V.L., Ashmore-Harris C., Lu W.Y., Thomson J.P., Jansen M.A., O’Duibhir E., Starkey Lewis P.J., Campana L., Aird R.E., Bate T.S., Fraser A.R., Campbell J.D., Oniscu G.C., Hay D.C., Callanan A, & Forbes S.J. (2022). Human biliary epithelial cells from discarded donor livers rescue bile duct structure and function in a mouse model of biliary disease. Cell Stem Cell, 29(3), 355-371.e10.

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Alkaline Phosphatase Activity Measurement

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Alkaline phosphatase (ALP) (Randox, UK) assay was applied to pDGSCs on modified and unmodified PBS scaffolds at 4, 10, and 20 days of incubation. In this assay, the absorbance was measured at 405 nm for time points of 0, 2, 4, 6, 8, 10, 12, and 14 min by Elisa Plate Reader. After the measurement of absorbance values, the slope of the absorbance versus time plot was used to calculate the alkaline phosphatase activity per min.
After the measurement of absorbance values, DNA was isolated from each sample and their DNA concentrations were measured using NanoDrop (Implen NanoPhotometer® P-Class, USA). To get normalized values, absorbance values were divided into DNA concentrations of each sample and the normalized ALP graph was constructed.

Abay N., Gurel Pekozer G., Ramazanoglu M, & Kose G.T. (2016). Bone Formation from Porcine Dental Germ Stem Cells on Surface Modified Polybutylene Succinate Scaffolds. Stem Cells International, 2016, 8792191.

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