Masshunter quantitative analysis software version 10

Data acquisition and processing were performed using Agilent MassHunter Quantitative Analysis software Version 10.1. SIMCA version 15.0.2 (Sartorious Stedim Biotech, Umea, Sweden) was used for statistical data analysis. SPSS was used for Student’s t test to compare data between two groups; P < 0.05 was considered statistically significant.

The pesticides (chlorfenapyr, diniconazole, and indoxacarb) were analyzed using an Agilent 7000D GC/TQ with a 7890 B gas chromatograph and a 7693A autosampler (Agilent Technologies, Santa Clara, CA, USA). Chromatographic separation was achieved on a DB-5MS UI column (0.25 mm I.D. × 30 m, 0.25 μm) (Agilent Technologies, Santa Clara, CA, USA). The oven temperature program was as follows: 200 °C hold for 0.1 min, increased to 250 °C at a rate of 40 °C/min, and increased to 300 °C at a rate of 60 °C/min and hold for 5 min. One microliter of the sample was injected in splitless mode. The total running time was 7.18 min. Helium gas was used as the carrier gas at a constant flow rate of 1.5 mL/min. Triple quadrupole MS was applied in dynamic multiple reaction monitoring mode with electron ionization at 70 eV. The GC-MS/MS parameters are summarized in Table 2. The ion source and transfer line temperatures were set at 250 and 280 °C, respectively. The MassHunter quantitative analysis software (version 10.1) (Agilent Technologies, Santa Clara, CA, USA) was used for data processing.