from Sigma-Aldrich, and their purity was higher than 99%. Mushroom
tyrosinase (50 kU) was from Sigma-Aldrich as a solid with a specific
activity of 2430 unit mg–1 (1 unit is defined as
the enzyme activity resulting in an increase in absorbance at 280
nm of 0.001 at pH 6.5 at 25 °C in a 3 mL reaction solution containing
diethylaminoethyl-cellulose (DEAE-cellulose, microgranular), carboxymethyl-cellulose
(CM-cellulose, microgranular), cellulose (fiber), hen egg-white lysozyme
(Mw: 14.4 kDa), and bovine serum albumin
(BSA, Mw: 66 kDa) were also from Sigma-Aldrich.