Total RNA was extracted from frozen cell pellets using Ambion Pure Link RNA Mini Kit (Life Technologies). Eight cDNA reactions were set-up from 1μg of total RNA per sample using High-Capacity cDNA RT Kit (Life Technologies). qPCR was performed on 384-well TaqMan hPSC Scorecard plates using Viia7 RUO software and Applied Biosystems ViiA7 instrument. The qPCR assay used in this study is commercially available as the TaqMan hPSC Scorecard panel (Life Technologies).
Taqman hpsc scorecard plates
Manufactured by Thermo Fisher Scientific
The TaqMan hPSC Scorecard plates are a laboratory equipment product designed for the analysis of human pluripotent stem cells (hPSCs). These plates contain a set of pre-designed TaqMan assays that target specific genes associated with the pluripotent state, lineage differentiation, and quality control markers. The plates enable researchers to assess the pluripotency, differentiation potential, and quality of hPSC cultures.
Automatically generated - may contain errors
Lab products found in correlation
Ambion purelink rna mini kit, by Thermo Fisher Scientific (2 mentions)
Viia7 instrument, by Thermo Fisher Scientific (2 mentions)
Taqman hpsc scorecard panel, by Thermo Fisher Scientific (2 mentions)
Viia 7 ruo software, by Thermo Fisher Scientific (2 mentions)
High capacity cdna rt kit, by Thermo Fisher Scientific (2 mentions)
2 protocols using taqman hpsc scorecard plates
1
Comprehensive hPSC Transcriptome Profiling
2
Comprehensive hPSC Transcriptome Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted from frozen cell pellets using Ambion Pure Link RNA Mini Kit (Life Technologies). Eight cDNA reactions were set-up from 1μg of total RNA per sample using High-Capacity cDNA RT Kit (Life Technologies). qPCR was performed on 384-well TaqMan hPSC Scorecard plates using Viia7 RUO software and Applied Biosystems ViiA7 instrument. The qPCR assay used in this study is commercially available as the TaqMan hPSC Scorecard panel (Life Technologies).
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