Protein extracts were prepared as previously described.45 (link) Overall, 10 μg of samples were electrophoresed and blotted to PVDF membrane (Bio-Rad, Hercules, CA, USA). Western blot was performed using primary antibodies against 15-lipoxygenase-1 (Mouse, Abcam, Cambridge, UK) caspase-3 (Rabbit, Cell Signaling, Danvers, MA, USA), cleaved form of caspase-3 (Rabbit, Cell Signaling), HSL (Rabbit, Cell signaling) and β-actin (Mouse, Santa Cruz Biotechnology, Dallas, TX, USA) and secondary anti-mouse and anti-rabbit horse-radish peroxidase antibodies (Cell Signaling Technology) as described previously.45 (link) The blots were visualized with SuperSignal West Dura Substrate (ThermoFisher Scientific).
Secondary anti mouse and anti rabbit horse radish peroxidase antibodies
Manufactured by Cell Signaling Technology
Sourced in United States
Secondary anti-mouse and anti-rabbit horse-radish peroxidase antibodies are laboratory reagents used in immunodetection techniques, such as Western blotting and ELISA. These antibodies are designed to recognize and bind to the primary antibodies that have been used to detect target proteins. The horse-radish peroxidase (HRP) enzyme conjugated to these secondary antibodies can then be used to generate a signal, allowing for the visualization and quantification of the target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west dura substrate, by Thermo Fisher Scientific (2 mentions)
Caspase 3, by Cell Signaling Technology (2 mentions)
15 lipoxygenase 1, by Abcam (2 mentions)
Pvdf membrane, by Bio-Rad (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
Cleaved form of caspase 3, by Cell Signaling Technology (1 mentions)
Tubulin, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3 asp175, by Cell Signaling Technology (1 mentions)
Keratin 14, by Cell Signaling Technology (1 mentions)
Phospho rip3, by Abcam (1 mentions)
Plin1, by Cell Signaling Technology (1 mentions)
Caspase 7, by Cell Signaling Technology (1 mentions)
2 protocols using secondary anti mouse and anti rabbit horse radish peroxidase antibodies
1
Western Blot Analysis of Lipoxygenase and Apoptosis Markers
2
Western Blot Analysis of Protein Markers
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Protein extracts were prepared as previously described22 (link). Overall, 10 μg of samples were electrophoresed and blotted to PVDF membrane (Bio-Rad, Hercules, CA, USA). Western blot was performed using primary antibodies against 15-lipoxygenase-1 (Mouse, Abcam, Cambridge, UK), PLIN1 (Rabbit, Cell Signaling, Danvers, MA, USA), Keratin 14 (Rabbit, Cell Signaling), phospho-RIP3(Rabbit, Abcam), RIP3(Rabbit, Cell signaling), ZBP1 (Rabbit, Cell Signaling), cleaved Caspase-3 (Asp175) (Rabbit, Cell Signaling), Caspase 3(Rabbit, Cell Signaling), Caspase 7(Rabbit, Cell Signaling), Tubulin (Cell Signaling), and β-actin (Mouse, Santa Cruz Biotechnology, Dallas, TX, USA) and secondary anti-mouse and anti-rabbit horse-radish peroxidase antibodies (Cell Signaling) as described previously. The blots were visualized with SuperSignal West Dura Substrate (ThermoFisher Scientific).
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