Thiazoyl blue tetrazolium bromide mtt

Minimum essential medium with Earl's salt and L-glutamine, RPMI 1640 medium with L-glutamine, fetal bovine serum, Pen-Strep (10 000 U/ml penicillin, 10 mg/ml streptomycin), trypsin 10× were supplied by GIBCO, Invitrogen (Auckland, New Zealand). JC-1 was purchased from Molecular Probes, Invitrogen (Oregon, USA). Dimethyl sulfoxide (DMSO), hydrochloric acid 37%, polyethylene glycol 400 (PEG 400) and 2-propanol were purchased from Merck (Hohenbrunn, Germany). Ethylene glycolbis(aminoethylether)-tetra-acetic acid (EGTA), ethylenediaminetetraacetic acid (EDTA), 3-(N-morpholino)propanesulfonic acid (MOPS), potassium chloride, potassium dihydrogen orthophosphate, sodium chloride, sodium hydrogen carbonate, disodium hydrogen orthophosphate anhydrous, sucrose and Tris were purchased Fisher Scientific (Leicestershire, UK). Thiazoyl blue tetrazolium bromide (MTT) was purchased from Amresco (Ohio, USA). Saline (0.9% sodium chloride) was obtained from Duopharma Sdn. Bhd. (Selangor, Malaysia). Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) was purchased from Sigma (Steinheim, Germany). Protein assay dye reagent concentrate was obtained from Bio-Rad Laboratories (California, USA). Complex I, Complex II, Complex IV and ATP synthase enzyme activity microplate assay kit were purchased from MitoSciences (Oregon, USA).

Minimum Essential Medium (MEM), 17β-E₂, E₁, EE and p-nitrophenyl phosphate (pNPP), Dimethyl sulfoxide (DMSO) and dextran-coated charcoal (DCC) were purchased from Sigma, USA. Fetal bovine serum (FBS), fetal calf serum (FCS), antibiotic-antimycotic and L-glutamine were obtained from Gibco, USA. Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F-12) was sourced from Nacalai Tesque, Japan. Glabridin was purchased from Wako, Japan. Phosphate buffered saline (PBS) was from OXOID, USA. Thiazoyl blue tetrazolium bromide (MTT) was obtained from Amresco, USA. Premarin was obtained from Wyeth Pharmaceuticals, USA. Receptor cofactor assay (RCAS) was purchased from Cosmo Bio Co., Ltd. All other chemicals were purchased from Merck, Germany.

Cell culture reagents including MEM medium, foetal bovine serum (FBS), penicillin-streptomycin, and trypsin were purchased from Gibco, Invitrogen (USA). 1,3-diphenylisobenzofuran (DPBF), isopropanol, Octanol-1, ethanol, PBS, Triton X-100, and polyethylene glycol 400 (PEG 400) were from Fisher Scientific (Malaysia). Cremophor-EL (CrEL), RPMI 1640 medium, dimethyl sulfoxide (DMSO), and fluorescein isothiocyanate dextran (FITC-dextran, 20 kD), were purchased from Sigma-Aldrich (USA). Thiazoyl blue tetrazolium bromide (MTT) was purchased from Amresco (USA).