Lk gam007

Western blot was performed as previously described (29 (link)). Briefly, sample lysates were resolved in SDS-PAGE and transferred onto PVDF membrane (Merck Millipore, Billerica, MA, USA). The PVDF membranes were incubated with the primary antibody JP2 (ab110056, Abcam, MA, USA, 1:1000) and GAPDH (HA-ET1702-66-200, HUABIO, Hangzhou, China), respectively. Then samples were incubated with HRP-conjugated secondary antibodies (LK-GAM007, LK-GAR007, LK-RAG007, MULTISCIENCES, Shanghai, China). Blots were developed using enhanced chemiluminescence reagents (Cat#. 32106, Pierce™, IL, USA).

Western blot was performed as previously described.^{47 (link)} Typically, sample lysates were resolved in SDS-PAGE and transferred onto PVDF membrane (Merck Millipore, Billerica, MA, USA). The PVDF membranes were incubated with the following primary antibodies (in addition to the above-mentioned): Errα, Sp1, IP3R, LTCC, Lamin B (Santa Cruz Biotechnology, CA, USA); RyR1, RyR2, RyR3 (Merck Millipore); SERCA2, GRP94, eIF2α, p-eIF2α, Bcl-2, Bax (Cell Signaling Technology); JPH1, JPH2, JPH3, JPH4, Mfn1, Mfn2, Pgc-1α (Abcam, Cambridge, MA, USA); GAPDH (Multisciences, Shanghai, China). Then samples were incubated with HRP-conjugated secondary antibodies (LK-GAM007, LK-GAR007, LK-RAG007, MULTISCIENCES). Blots were developed using enhanced chemiluminescence reagents (Pierce). Coimmunoprecipitates were analyzed by immunoblotting, using either anti-JPH3 or anti-RyR1, 2, 3 antibodies to detect the interaction between JPH3 and 3 RyR subtypes.