Naringinase from Aspergillus aculeatus/Aspergillus niger (Novozyme NS 33117) was kindly donated by Novozyme. Naringin (≥95% HPLC), limonin (>90% HPLC), p-nitrophenyl-α-l -rhamnopyranoside (pNPR), and p-nitrophenyl-β-d -glucoside (pNPG) were purchased from Sigma-Aldrich, Santiago, Chile. Acetonitrile, octyl-sepharose 4 Fast Flow, butyl-sepharose 4 Fast Flow, and sodium metaperiodate were purchased from Merck, Santiago, Chile. All other chemicals were of analytical grade. Grapefruits were purchased from the local market.
P nitrophenyl α l rhamnopyranoside
Manufactured by Merck Group
Sourced in Chile, United States
P-nitrophenyl-α-l-rhamnopyranoside is a chemical compound used as a substrate for enzymatic assays. It is a modified sugar molecule that can be used to measure the activity of specific enzymes.
Automatically generated - may contain errors
Lab products found in correlation
P nitrophenyl β d glucopyranoside, by Merck Group (2 mentions)
Sodium metaperiodate, by Merck Group (1 mentions)
Limonin, by Merck Group (1 mentions)
P nitrophenyl β d glucoside, by Merck Group (1 mentions)
Naringin, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Isoquercetin, by Merck Group (1 mentions)
Isoamyl alcohol, by Merck Group (1 mentions)
Narcissin, by Extrasynthese (1 mentions)
Hesperidin, by Merck Group (1 mentions)
4 methylumbelliferone, by Merck Group (1 mentions)
2 phenylethanol, by Merck Group (1 mentions)
N propanol, by Merck Group (1 mentions)
Hesperidin methyl chalcone, by Merck Group (1 mentions)
Lichrosolv, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Isopropanol, by Merck Group (1 mentions)
Resorcinol, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
3 protocols using p nitrophenyl α l rhamnopyranoside
1
Purification and Characterization of Naringinase
2
Enzymatic Activity of Plant-Derived Compounds
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4-methylumbelliferone, hesperidin, rutin, hesperidin methylchalcone, isoquercetin, resorcinol, phloroglucinol, methanol, ethanol, butanol, n-propanol, isopropanol, isoamyl alcohol, 2-phenylethanol, p-Nitrophenyl-β-d -glucopyranoside and p-Nitrophenyl-α-l -rhamnopyranoside were purchased in Sigma Chemical (Sigma, St. Louis, MO, USA). Narcissin, tulipanin and myrtillin were purchased in Extrasynthese company (Extrasynthese, Genay Cedex, France). HPLC-grade methanol (LiChrosolv®) was obtained from Merck (Merck, Darmstadt, Germany). The 4-methylumbelliferyl-rutinoside reagent was synthesized as described in Mazzaferro et al. [29 (link)].
3
Enzymatic Activity Assays in Permeabilized Cells
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Enzymatic assays were performed as described by Maturano et al. (2016) using p-nitrophenyl-β-d-glucopyranoside (Sigma N7006) , p-nitrophenyl-α-l-arabinofuranoside (Sigma N3641) and p-nitrophenyl-α-l-rhamnopyranoside (Sigma N7763) as substrates for β-glucopyranosidase, α-arabinofuranosidase and α-rhamnopyranosidase enzymes, respectively. The enzyme assays were conducted in permeabilized cells according to a procedure optimized in our laboratory (Maturano et al. 2016 ). The enzymatic activity was expressed as unit (U) which was equivalent to µmol of p-nitrophenyl released per minute and mL.
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