FAMEs were prepared according to the IUPAC Standard Method [32 ]. The oil samples in hexane (50 mg·2 mL−1) were transesterified using 250 µL KOH/MeOH 2 N and the FAMEs formed were analyzed using a 7890B Agilent gas chromatograph (Agilent Technologies, USA) equipped with a SP2380 polar capillary column (poly (90% biscyanopropyl–10% cyanopropylphenyl-siloxane, 60 m × 0.25 mm i.d.; 0.20 μm film thickness) and a flame ionization detector (FID). The injector and detector temperatures were maintained at 225 and 250 °C, respectively. Hydrogen was used as carrier gas at a flow rate of 1.0 mL·min−1. The oven temperature was set at 165 °C and increased to 230 °C at 3 °C·min−1 maintaining this temperature for 2 minutes. The injection volume was 1 μL.
Sp2380
Manufactured by Agilent Technologies
Sourced in United States
The SP2380 is a high-performance liquid chromatography (HPLC) system manufactured by Agilent Technologies. It is designed to perform efficient and reliable separation and analysis of a wide range of chemical compounds. The SP2380 features advanced technology and precise instrumentation to deliver accurate and reproducible results.
Automatically generated - may contain errors
3 protocols using sp2380
1
Fatty Acid Methyl Ester Analysis by GC-FID
2
GC-MS Analysis of Permethylated Polysaccharides
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Permethylated polysaccharides were characterized by a gas chromatograph (HP-Agilent 6850) using a 530 μm capillary column (SP 2380). The injector temperature is 260 °C with a constant flow of 4 ml·min−1 nitrogen carrier gas. Column temperature was held at 165 °C for 4 min and ramped at 2.5 °C·min to 225 °C and held for 3 min. After separation in capillary column, methylated derivatives were analyzed by a mass spectrometer (Agilent 5975C, Agilent technologies Inc., Santa Clara, CA, USA).
3
Glycosyl Linkage Analysis of Polysaccharides
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EPS matrix (2 mg) or CW preparation of S. indica or B. sorokiniana were ground with a stainless steel bead (5 mm) using a TissueLyser mill at 30 Hz for 1 minute.
The powdered material was subjected to glycosyl linkage analysis as described (Liu et al., 2015) . Briefly, a methylation reaction was performed using NaOH/DMSO. The methylated compounds were hydrolyzed in 1 M trifluoroacetic acid, reduced using sodium borodeuteride (ACROS Organics, cat.no. 194950050 ) and per-o-acetylated.
The resulting partially methylated alditol acetates were analyzed using an Agilent 5977A GC/MSD System equipped with a SP-2380 Fused Silica Capillary Column was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 10, 2021. ; https://doi.org/10.1101/2021.05.10.443455 doi: bioRxiv preprint (Supelco). The glycosidic linkages were assigned based on retention time and mass spectrum fragmentation patterns compared to the CCRC spectral database (https://www.ccrc.uga.edu/specdb/ms/pmaa/pframe.html).
The powdered material was subjected to glycosyl linkage analysis as described (Liu et al., 2015) . Briefly, a methylation reaction was performed using NaOH/DMSO. The methylated compounds were hydrolyzed in 1 M trifluoroacetic acid, reduced using sodium borodeuteride (ACROS Organics, cat.no. 194950050 ) and per-o-acetylated.
The resulting partially methylated alditol acetates were analyzed using an Agilent 5977A GC/MSD System equipped with a SP-2380 Fused Silica Capillary Column was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this preprint (which this version posted May 10, 2021. ; https://doi.org/10.1101/2021.05.10.443455 doi: bioRxiv preprint (Supelco). The glycosidic linkages were assigned based on retention time and mass spectrum fragmentation patterns compared to the CCRC spectral database (https://www.ccrc.uga.edu/specdb/ms/pmaa/pframe.html).
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