Axiocam hrc digital microscope camera

Cell adhesion and tube formation experiments were performed as previously described [32 (link)]. After treatments, BMECs (1,000 cells) were added to 24-well plates coated with fibronectin. BMECs that had adhered to fibronectin were counted under a phase-contrast microscope. Experiments were performed in triplicate.
BMECs (100,000 cells per well) were seeded into 24-well plates (100,000 cells per well) coated with 200 μL of growth factor-reduced Matrigel from BD Biosciences. Branches were quantified after a 24 h incubation at 37°C. Images were taken with the SteREO Discovery.V20 microscope from Carl Zeiss (Jena, Germany), equipped with a plan apochromat 1.0x and the AxioCam HRc digital microscope camera from Carl Zeiss, automated with the AxioVision Rel.4.8 software from Carl Zeiss. Experiments were performed in triplicate.

Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays were performed using the TransDetect In Situ Fluorescein TUNEL Cell Apoptosis Detection Kit (FA201-02) (Beijing, China). Briefly, cells were plated onto cover glasses in 6-well plates. After treatments, TUNEL experiments were performed and images were taken with the SteREO Discovery.V20 microscope from Carl Zeiss (Jena, Germany), equipped with a plan apochromat 1.0x and the AxioCam HRc digital microscope camera from Carl Zeiss, automated with the AxioVision Rel.4.8 software (Carl Zeiss).
Cell apoptosis was also analyzed using the Annexin V-FITC Apoptosis Kit (K101) from Biovision (Milpitas, CA, USA), in accordance with the manufacturer's instructions. After labeling, the cells were analyzed using a MoFlo Astrios cell sorter from Beckman-Coulter.