Rabbit anti pgc 1α

Manufactured by Novus Biologicals

Sourced in United States, Canada

Rabbit anti-PGC-1α is a primary antibody that recognizes the Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-Alpha (PGC-1α) protein. PGC-1α is a transcriptional coactivator that plays a key role in regulating cellular energy metabolism.

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Lab products found in correlation

Mouse anti beta 3 tubulin, by Abcam (1 mentions) Cy3 goat anti rabbit igg h l, by Jackson ImmunoResearch (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Isotype control antibody, by Jackson ImmunoResearch (1 mentions) Anti ki67, by Thermo Fisher Scientific (1 mentions) Vectastain abc system, by Vector Laboratories (1 mentions) Diva decloaker, by Biocare Medical (1 mentions) Rabbit anti gpr81, by Abnova (1 mentions) Rabbit anti mct1, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-PGC-1α PGC-1α

2 protocols using rabbit anti pgc 1α

Immunofluorescence Staining of Neural and Metabolic Markers

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Immunofluorescence staining was performed on frozen sections. In brief, sections were rinsed in PBS and treated with 0.3 % Triton X-100 in PBS to disrupt the membranes. After blocking with 10 % normal goat serum for 1 h to reduce nonspecific staining, sections were incubated with the primary antibodies, mouse anti-beta III tubulin (Abcam, USA, 1:100) and rabbit anti-PGC-1α (Novus, USA, 1:150) overnight at 4 °C for 24 h. After rinsing in PBS, the secondary antibodies, Alexa Fluor 488 goat anti-mouse IgG (H + L) (Jackson, USA, 1:600) and Cy3 goat anti-rabbit IgG (H + L) (Jackson, USA, 1:800), were added onto sections in 5 % blocking solution, to incubate at room temperature for 30 min. Then, the sections were counterstained with DAPI (Sigma, USA), and imaged using a Leica DFC310 FX microscope (Leica, Japan).

Hu J., Lang Y., Cao Y., Zhang T, & Lu H. (2015). The Neuroprotective Effect of Tetramethylpyrazine Against Contusive Spinal Cord Injury by Activating PGC-1α in Rats. Neurochemical Research, 40(7), 1393-1401.

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Immunohistochemical Analysis of Metabolic Markers

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Tissues were either fixed in 4% formalin then embedded in paraffin or snap frozen in liquid nitrogen and embedded in ornithine carbamyl transferase medium and sectioned. Paraffin embedded sections were deparaffinized with xylene and rehydrated with ethanol. Antigen retrieval was performed with diva decloaker (Biocare Medical, Concord, CA) in a steamer for 20 minutes. Endogenous peroxidase was blocked with 4% H₂O₂ and protein-blocked with 4% fish gelatin. Frozen sections were fixed in acetone, briefly air dried, and blocked with 4% fish gelatin for 30 minutes. Primary antibodies were incubated overnight at 4°C and included: rabbit anti-GPR81 (Abnova, Walnut, CA), 1:50 dilution; rabbit anti-MCT1 (Santa Cruz Biotechnology, Santa Cruz, CA), 1:50 dilution; rabbit anti-PGC1α (Novus Biologicals, Littleton, CO), 1:50 dilution; anti-Ki67 (Thermo Fischer Scientific, Waltham, MA). Negative controls were done using isotype control antibodies (Jackson ImmunoResearch, West Grove, PA). Following washes, VECTASTAIN^® ABC systems (Vector laboratories, Burlingame, CA) was added to paraffin sections per manufacturer protocol, developed with 3,3-diaminobenzidine substrate, counterstained with hematoxylin and mounted with water soluble mounting media. Frozen sections were developed with fluorophore-conjugated secondary antibody, as described above.

Roland C.L., Arumugam T., Deng D., Liu S.H., Philip B., Gomez S., Burns W.R., Ramachandran V., Wang H., Cruz-Monserrate Z, & Logsdon C.D. (2014). Cell surface lactate receptor GPR81 is crucial for cancer cell survival. Cancer research, 74(18), 5301-5310.

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