A total protein extraction kit (Solarbio) was used to extract bladder tissues or cells proteins. The protein samples were subjected to sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (EMD Millipore). The membranes were incubated with primary antibodies (bFGF (1:1000, Bioss), HGF (1:1000, Bioss), VEGF-A (1:1000, Bioss), AKT (1:2000, Proteintech), pAKT (1:2000, Proteintech), GSK-3β(1:1000, Proteintech), pGSK-3β(1:2000, Proteintech), and GAPDH (1:2000, Proteintech)) overnight at 4 °C after blocked with 5% non-fat milk, then followed by a secondary horseradish peroxidase-conjugated antibody (anti-rabbit or anti-mouse (1:5000, Cell Signaling)) for 2 h at room temperature. Enhanced chemiluminescence system (ECL kit) was used to detect the immunoblot signals, which were quantified by scanning densitometry using the ImageJ analysis system (NIH).
Hepatocyte growth factor (hgf)
Manufactured by Bioss Antibodies
Sourced in China
HGF is a laboratory reagent used in research applications. It is a recombinant human hepatocyte growth factor that can be used in cell culture studies. The core function of HGF is to stimulate the proliferation and migration of various cell types.
Automatically generated - may contain errors
Lab products found in correlation
Basic fibroblast growth factor (bfgf), by Bioss Antibodies (1 mentions)
P gsk 3β, by Proteintech (1 mentions)
Vegfa, by Bioss Antibodies (1 mentions)
Gapdh, by Proteintech (1 mentions)
Polyvinylidene difluoride membrane, by Merck Group (1 mentions)
P akt, by Proteintech (1 mentions)
Gsk3β, by Proteintech (1 mentions)
Insulin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using hepatocyte growth factor (hgf)
1
Western Blot Analysis of Bladder Proteins
2
Immunohistochemical Analysis of Organ Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse liver, pancreas, kidney sections were dewaxed and rehydrated, and then blocked with 5%BSA in PBS-Tx-100 /0.05% for 1h at room temperature. Subsequently, the sections were incubated with hepatocyte growth factor (HGF) (1:150, Bioss, China), insulin (1:500, Santa Cruz, USA), renin (1:150, Bioss, China) primary antibodies overnight at 4°C. After being washed with PBSTx buffer several times, the sections were exposed to corresponding antibodies (1:500, Boster, China), and then re-exposed to hydrogen peroxidase enzyme-labeled diaminobenzidine (DAB) dye before re-dyeing with haematoxylin to cytoblast [14, 15] .
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