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Jq1 s7110

Manufactured by Selleck Chemicals
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Sourced in China

JQ1(S7110) is a chemical compound used in laboratory settings. It is a selective inhibitor of the bromodomain and extra-terminal (BET) family of proteins, which play a role in regulating gene expression. The core function of JQ1(S7110) is to modulate the activity of BET proteins, which can be useful in various areas of scientific research.

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Lab products found in correlation

Ab200342, by Abcam (1 mentions) Ab52757, by Abcam (1 mentions) I bet762, by Selleck Chemicals (1 mentions) Flag tag (flag), by Merck Group (1 mentions) Hct116, by American Type Culture Collection (1 mentions) Cleaved caspase 3 antibody 9661, by Cell Signaling Technology (1 mentions) Alexa fluor 594 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Crystal violet, by Beyotime (1 mentions) Hematoxylin and eosin staining kit, by Beyotime (1 mentions) Actin tracker green, by Beyotime (1 mentions)

4 protocols using jq1 s7110

1

Cell Culture and Western Blot

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SW480 and HCT116 was obtained from the American Type Culture Collection (ATCC). SW480 was cultured in L-15 medium (GENOM, GNM41300) supplemented with 10% bovine serum at 37°C without CO2. HCT116 was cultured in McCOY’s 5A medium (GENOM, GNM16600) supplemented with 10% bovine serum at 37°C with 5% CO2. The following antibodies were used for Western blot and CO-IP: HSF1 (ab52757, Abcam), β-actin (4970 L, CST), Flag (F1804, Sigma), β-catenin (8480S, CST), HuR (Abcam, ab200342). JQ1(S7110) and I-BET-762 (S7189) was purchased from Selleck.
Ren X., Zhang L., Ma X., Li J, & Lu Z. (2022). Integrated bioinformatics and experiments reveal the roles and driving forces for HSF1 in colorectal cancer. Bioengineered, 13(2), 2536-2552.
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2

JQ1 and I-BET-762 Compound Treatments

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JQ1 (S7110) and I-BET-762 (S7189) were purchased from Selleck (Shanghai, China). 2 × 105 cells were seeded in 6-well plates and added JQ1-1uM or I-BET-762 2 µM for 24 h.
Wang R., Liu N., Li G., Liu J., Ma X., Liu X, & Li J. (2023). Pan-cancer analysis of super enhancer-induced PRR7-AS1 as a potential prognostic and immunological biomarker. Frontiers in Genetics, 14, 1160599.
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3

Antibody and Compound Validation Protocol

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HAT1 antibody (ab193097), KDM5C antibody (ab34718), and BRD4 antibody (ab128874) were purchased from Abcam. GAPDH antibody (5174S), AR antibody (5153S), H4K5ac antibody (8647S), Cleaved caspase 3 antibody (9661S), and Ki‐67 antibody (9027S) were purchased from Cell Signaling Technology. Ascorbate (S4245) and JQ1 (S7110) were purchased from Selleckchem company (Shanghai, China).
Hong Z., Xiang Z., Zhang P., Wu Q., Xu C., Wang X., Shi G., Hong Z, & Wu D. (2021). Histone acetyltransferase 1 upregulates androgen receptor expression to modulate CRPC cell resistance to enzalutamide. Clinical and Translational Medicine, 11(7), e495.
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4

Investigating GPX8 and Focal Adhesion Signaling

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The primary antibody of GPX8 (16846‐1‐AP) was acquired from Proteintech Group (Rosemont, IL, USA). The primary antibodies against p‐FAK (Tyr397, #3283), FAK (#71433), p‐Paxillin (Tyr118, #2541), Paxillin (#2542), and GAPDH (#5174) were obtained from Cell Signaling Technology (Beverly, MA, USA). The secondary HRP‐linked antibody (anti‐rabbit IgG, #7074) was purchased from Cell Signaling Technology. The Alexa Fluor® 594 conjugated secondary antibody (#A‐11001) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Actin‐Tracker Green, Hematoxylin and Eosin Staining Kit, and Crystal violet were acquired from Beyotime Biotechnology Corporation (Shanghai, China). JQ1(S7110) and PFI‐1(S1216) were purchased from Selleck Chemicals (Houston, TX, USA).
Xu Y., Yuan L., Jiang X., Su M., Huang M., Chen Y., Zhang L., Chen X., Zhu H, & Lu J. (2022). Glutathione peroxidase 8 expression on cancer cells and cancer‐associated fibroblasts facilitates lung cancer metastasis. MedComm, 3(3), e152.
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