Pb7wg2d vector

Manufactured by Thermo Fisher Scientific

Sourced in Belgium

The PB7WG2D vector is a plasmid vector used for gene expression and recombinant protein production. It contains a promoter, a multiple cloning site, and a selection marker. The vector's core function is to facilitate the cloning and expression of target genes in various host cells.

Automatically generated - may contain errors

Lab products found in correlation

Lr clonase enzyme, by Thermo Fisher Scientific (2 mentions) Eporator, by Eppendorf (2 mentions) Pentr d topo vector, by Thermo Fisher Scientific (1 mentions) Pdonr221 vector, by Thermo Fisher Scientific (1 mentions) Bp clonase enzyme, by Thermo Fisher Scientific (1 mentions)

2 protocols using pb7wg2d vector

Overexpression of RsCLER2, 19, and 41 in Radish

Check if the same lab product or an alternative is used in the 5 most similar protocols

PCR-fragments amplified on R. sativus DNA with primers for full-length CDS of RsCLER2, 19 and 41 were cloned to pENTR/D-TOPO vector (Invitrogen, USA) and after that transferred to pB7WG2D vector (Ghent, Belgium) for overexpression using the LR Clonase enzyme (Invitrogen, USA). This vector contains GFP gene under constitutive promoter for transgenic organs selection.
Constructs were introduced into Agrobacterium rhizogenes MSU440 strain via electroporation using Eppendorf Eporator® (Eppendorf, Germany).

Gancheva M.S., Dodueva I.E., Lebedeva M.A., Tvorogova V.E., Tkachenko A.A, & Lutova L.A. (2016). Identification, expression, and functional analysis of CLE genes in radish (Raphanus sativus L.) storage root. BMC Plant Biology, 16(Suppl 1), 7.

+ Open protocol

+ Expand

Cloning and Overexpression of RsCLE41-1

Check if the same lab product or an alternative is used in the 5 most similar protocols

PCR fragments amplified on R. sativus DNA with primers for the full-length CDS of RsCLE41-1 were cloned to pDONR221 vector (Ghent, Belgium) using the BP Clonase enzyme (Invitrogen, Waltham, MA, USA) and after that transferred to pB7WG2D vector (Ghent, Belgium) for overexpression using the LR Clonase enzyme (Invitrogen, Waltham, MA, USA). Constructs were introduced into Agrobacterium rhizogenes Arqua strain via electroporation using Eppendorf Eporator^® (Eppendorf, Hamburg, Germany).

Kuznetsova K., Dodueva I., Gancheva M, & Lutova L. (2022). Transcriptomic Analysis of Radish (Raphanus sativus L.) Roots with CLE41 Overexpression. Plants, 11(16), 2163.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!