phospho‐S6K1 (T389, Cat#: 9205), phospho‐S6K1 (T421/S424, Cat#: 9204), phos‐rpS6 (S235/236, Cat#: 4858), phos‐rpS6 (S240/244, Cat#: 5364), rpS6 (Cat#: 2317), 4E‐BP1 (Cat#: 9644), phos‐mTOR (S2448, Cat#: 5536), mTOR (Cat#: 2972), phos‐Akt (S473, Cat#: 4060), phos‐Akt (T308, Cat#: 5106), Akt (pan, Cat#: 4691), phos‐Akt1 (S473, Cat#: 9018), Akt1 (Cat#: 2967) were from Cell Signaling Technology. glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH; Cat#: sc‐32233) and S6K1 (Cat#: sc‐230) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). IRDye 800CW Goat anti‐Mouse IgG (Cat#: 926‐32210) and IRDye 680LT Goat anti‐Rabbit IgG (Cat#: 926‐68021) were from LI‐COR Biosciences.
Phos mtor
Manufactured by Cell Signaling Technology
Sourced in United States, China
Phos-mTOR is a primary antibody that specifically recognizes the phosphorylated form of the mammalian target of rapamycin (mTOR) protein. mTOR is a serine/threonine protein kinase that plays a central role in the regulation of cell growth, proliferation, and metabolism. The Phos-mTOR antibody can be used to detect the activated, phosphorylated state of mTOR, which is an important marker for monitoring mTOR signaling pathways.
Automatically generated - may contain errors
Lab products found in correlation
Phospho s6k1, by Cell Signaling Technology (1 mentions)
Phos rps6, by Cell Signaling Technology (1 mentions)
Irdye 680lt goat anti rabbit igg, by LI COR (1 mentions)
Pan akt, by Cell Signaling Technology (1 mentions)
Irdye 800cw goat anti mouse igg, by LI COR (1 mentions)
4e bp1, by Cell Signaling Technology (1 mentions)
Phos akt, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase gapdh, by Santa Cruz Biotechnology (1 mentions)
Phos p70s6k, by Cell Signaling Technology (1 mentions)
β actin ab, by Cell Signaling Technology (1 mentions)
Hmgb1, by Cell Signaling Technology (1 mentions)
Hif 1α, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Nf κb, by Cell Signaling Technology (1 mentions)
2 protocols using phos mtor
1
Quantifying mTOR Signaling Pathway
2
Liver Protein Extraction and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was extracted from liver tissue or cell cultures, as described53 (link). Monoclonal rabbit anti-mouse ATF3 (Santa Cruz Biotechnology, Shanghai, China), phos-mTOR, mTOR, phos-p70S6K, p70S6K, HMGB1, TLR4, NF-κB, HIF-1α, PHD1, Bcl-2, Bcl-xl, and β-actin Abs (Cell Signaling Technology, San Diego, CA) were used. The relative quantities of proteins were determined by densitometer, and expressed in absorbance units (AU).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!