Platencin

Sodium acetate (1-¹³C and 2-¹³C, each 99% enriched) was purchased from Cambridge Isotope Laboratories, Inc. Cerulenin, platencin, platensimycin and 8-amino-7-oxononanoate were purchased from Cayman Chemical. All other chemicals were purchased from Fisher Scientific or Sigma Aldrich. The bacterial strains are given in Table 1. The B. subtilis strains are all derivatives of strain 168 whereas the E. coli strains are derivatives of the wild type K-12 strains MC1061 or MG1655. The B. subtilis biotin overproducing strain BI274 and BI304 (derivatives of strain PY79 with constitutive expression of the bio operon) (Bower, 2000 ) was from the American Type Culture Collection (accession 55575 and 55573, respectively). The cer-20 derivative of B. subtilis 168 that is partially resistant to Cerulenin (Schujman et al., 2001 (link)) was from the Bacillus Genetics Stock Center (accession 1A577).