Formamidopyrimidine dna glycosylase

DNA repair enzymes uracil-DNA glycosylase (UDG,
#M0280S), human apurinic/apyrimidinic endonuclease 1 (APE1, #M0282S),
formamidopyrimidine DNA glycosylase (FPG, #M0240S) and E. coli DNA ligase (ligase, #M0205S), endonuclease
III (Endo III, #M0268S), and TTH endonuclease IV (TTH, #M0294S) were
obtained from New England Biolabs (NEB). Mismatch-specific endonuclease
I (MSE, #M0678S) and restriction enzymes HpaII (#R0171S) and HaeIII
(#R0108S) were also purchased from NEB. Human 8-oxoguanine DNA glycosylase
(hOGG1, #NBP1-45318-0.1mg) and DNA polymerase β (Pol β,
#NBP1-72434-0.5mg) were purchased from Novus Biologicals. Our hybrid
thymine DNA glycosylase (hyTDG) was prepared as previously described.^{18 (link)}