HEK293T stable cell lines expressing variants of InterTag and InterCatch with different linkers were cultured separately in 6 well chambers (Falcon, 353046). Upon standard passaging, cells were dissociated with 200 μL of Trypsin-EDTA (0.05%, Gibco, 25300054) and seeded at a density of 1.00×105 cells/mL into Nunc™ Lab-Tek™ Chambered Coverglass (155361). The following day, InterTag and InterCatch monolayers were trypsinized and resuspended into 500 μL DMEM. InterTag and InterCatch cell suspensions were mixed and transferred to an orbital shaker in the humidified incubator for 45 minutes at 100 rpm. Cell suspensions were then centrifuged and pelleted (~200 g for 5 min). The media was aspirated and refreshed with another 500 μL of DMEM. Cells were gently resuspended via pipetting and seeded onto fibronectin- coated wells (0.5% in dPBS, Sigma-Aldrich, F1141) and incubated for 1 hour before imaging.
6 well chambers
Manufactured by BD
The 6 well chambers are a versatile laboratory equipment designed to facilitate various cell culture and experimental applications. These chambers provide a controlled environment for culturing cells and performing various assays. The core function of the 6 well chambers is to provide a standardized, multi-well format for culturing and analyzing cells.
Automatically generated - may contain errors
2 protocols using 6 well chambers
1
Proximity Assay for Interacting Proteins
2
Interactome Protein Complex Formation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HEK293T stable cell lines expressing variants of InterTag and InterCatch with different linkers were cultured separately in 6 well chambers (Falcon, 353046). Upon standard passaging, cells were dissociated with 200 µL of Trypsin-EDTA (0.05%, Gibco, 25300054) and seeded at a density of 1.00x10 5 cells/mL into Nunc™ Lab-Tek™ Chambered Coverglass (155361). The following day, InterTag and InterCatch monolayers were trypsinized and resuspended into 500 µL DMEM. InterTag and InterCatch cell suspensions were mixed and transferred to an orbital shaker in the humidified incubator for 45 minutes at 100 rpm. Cell suspensions were then centrifuged and pelleted (~200 g for 5 min). The media was aspirated and refreshed with another 500 µL of DMEM. Cells were gently resuspended via pipetting and seeded onto fibronectin-coated wells (0.5% in dPBS, Sigma-Aldrich, F1141) and incubated for 1 hour before imaging.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!