Fm1 43x

Manufactured by Thermo Fisher Scientific

Sourced in United States

FM1-43X is a fluorescent styryl dye used to label cell membranes and study endocytosis. It is a lipophilic, water-soluble compound that becomes highly fluorescent when incorporated into lipid bilayers.

Automatically generated - may contain errors

Lab products found in correlation

M0387, by Merck Group (1 mentions) Ds ri1, by Nikon (1 mentions) Smz1500, by Nikon (1 mentions) Fast blue, by Polysciences (1 mentions) Gelfoam 12 7 mm, by Pfizer (1 mentions)

2 protocols using fm1 43x

Visualizing Neuromasts in Larval Zebrafish

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescent dye FM1-43X (ThermoFisher), a fixable analog of FM1-43, was used to stain functional larval lateral line neuromast hair cells.⁶¹ (link) A fresh FM1-43X stock solution dissolved in distilled water (817μM) was diluted to 3μM final concentration in E3 embryo medium. 3dpf larvae were stained in 3μM FM1-43X for 20 seconds, then immediately anesthetized in Syncaine (MS-222, Syndel) diluted in E3 embryo medium for 10 minutes and mounted laterally onto a glass slide in 4.0% methyl cellulose (M0387, Sigma Aldrich). Fish were immediately imaged using a Nikon dissecting microscope (Nikon SMZ1500) and camera (Nikon DS-Ri1) equipped with a fluorescent lamp illuminator (X-Cite Series 120Q) fitted with an appropriate emission filter (Ex 450-490 nm, Em >500 nm). Anterior Lateral Line neuromasts of the head were counted on only one side of each larva, in the stereotyped SO, O, OC, D, M, IO, OP, and MI regions of the head (as described by Harris et al.¹⁰⁶ (link)). Posterior Lateral Line neuromasts of the trunk and tail (P1-P9, as described by Harris et al.¹⁰⁶ (link)) were scored along the horizontal myoseptum, including neuromasts on both sides of the tail as these were all clearly visible with a minor adjustment in focus. All larvae were scored blind to genotype, and scores were subsequently matched to genotypes post-scoring.

Zúñiga Mouret R., Greenbaum J.P., Doll H.M., Brody E.M., Iacobucci E.L., Roland N.C., Simamora R.C., Ruiz I., Seymour R., Ludwick L., Krawitz J.A., Groneberg A.H., Marques J.C., Laborde A., Rajan G., Del Bene F., Orger M.B, & Jain R.A. (2024). The adaptor protein 2 (AP2) complex modulates habituation and behavioral selection across multiple pathways and time windows. iScience, 27(4), 109455.

+ Open protocol

+ Expand

Corneal Tracer Application via Iontophoresis

Check if the same lab product or an alternative is used in the 5 most similar protocols

At 72 h prior to LGE or sham surgery tracers were applied to the cornea, including FluoroGold (FG, 3% in Na⁺Cl⁻; Fluorochrome, Denver, CO, USA), Fast Blue (FB, 5% in Na⁺Cl⁻; PolySciences, Warrington, PA), FM-143x (FM, 5% in NaCl, ThermoFisher Scientific, Waltham, MA, USA) and 1,1′-dioctadecyl-3,3,3′3,3′ -tetramethyl-indocarbocyanine perchlorate (DiI, 5% in 20% DMSO in H₂O, ThermoFisher Scientific, Waltham, MA, USA). Under isoflurane anesthesia, a DC current (7 μA for 10 min) (Kumar et al., 2017 ) was passed between a copper wire in a capillary tube filled with either a single tracer or a combination of two (FG/FM143x, FB/FM143x, FG/DiI) and a copper cathode placed in the tail (Fig. 1A). An absorbent gelatin sponge (Gelfoam 12–7 mm; Pfizer Pharmaceuticals, New York, NY) was cut, inserted into the capillary tube, and placed in contact with both the cornea and the tracer solution within the capillary tube, thus allowing the current to pass through to the cornea (Cassagne et al., 2016 (link)). In preliminary studies, iontophoresis did not produce signs of epithelial damage as evidenced by an absence of corneal fluorescein staining.

Sullivan C., Lee J., Bushey W., Demers D., Dinsdale S., Lowe K., Olmeda J, & Meng I.D. (2022). Evidence for a phenotypic switch in corneal afferents after lacrimal gland excision. Experimental eye research, 218, 109005.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!