Blastocyst cell counting46 (link) was performed by fixing blastocysts in 4% (w/v) paraformaldehyde and incubating them at 4 °C overnight in rabbit anti-Oct4 antibody (1:100; Santa Cruz) or for 1 h at 37 °C in mouse anti-Cdx2 antibody (1:100; BioGenex, USA), followed by 1 h at 37 °C in Alexa 488-conjugated anti-rabbit IgG (Invitrogen) or Alexa 594-conjugated anti-mouse IgG (Invitrogen) respectively. Cells stained with Alexa 488 were scored as Oct4-positive (pluriblasts) and those with Alexa 594, as Cdx2-positive (trophoblasts).
Mouse anti cdx2 antibody
Manufactured by BioGenex
Sourced in United States
The Mouse anti-Cdx2 antibody is a laboratory reagent designed for the detection and identification of the Cdx2 protein in biological samples. Cdx2 is a transcription factor that plays a crucial role in the development and differentiation of intestinal epithelial cells. This antibody can be used in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry, to investigate the expression and localization of Cdx2 in cells and tissues.
Automatically generated - may contain errors
2 protocols using mouse anti cdx2 antibody
1
Blastocyst Cell Counting by Immunofluorescence
2
Immunofluorescence Staining of Embryos
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Immunofluorescence staining was performed as described previously (Lu, Zhang et al. 2019) .
Briefly, embryos were washed three times and then fixed with 4% paraformaldehyde in PBS for 30 min at RT. They were then permeabilized with 1% Triton X-100 in PBS, and incubated in blocking solution (1% bovine serum albumin in PBS) for 1 h at RT. For immunolabeling, embryos were incubated with a rabbit anti-H3K9 me3 antibody (Abcam, Cambridge, MA, USA) or a mouse anti-alpha tubulin (acetyl K40) antibody (Abcam, Cambridge, MA, USA)
or a mouse anti-CDX2 antibody (BioGenex, Fremont, California, USA) overnight at 4°C. On the second day, according to the species of primary antibody raised, the embryos were
Briefly, embryos were washed three times and then fixed with 4% paraformaldehyde in PBS for 30 min at RT. They were then permeabilized with 1% Triton X-100 in PBS, and incubated in blocking solution (1% bovine serum albumin in PBS) for 1 h at RT. For immunolabeling, embryos were incubated with a rabbit anti-H3K9 me3 antibody (Abcam, Cambridge, MA, USA) or a mouse anti-alpha tubulin (acetyl K40) antibody (Abcam, Cambridge, MA, USA)
or a mouse anti-CDX2 antibody (BioGenex, Fremont, California, USA) overnight at 4°C. On the second day, according to the species of primary antibody raised, the embryos were
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